Huang X Y, Hirsh D
Department of Developmental Biology, Synergen, Inc., Boulder, CO 80301.
Proc Natl Acad Sci U S A. 1989 Nov;86(22):8640-4. doi: 10.1073/pnas.86.22.8640.
In the nematode Caenorhabditis elegans, the 22-nucleotide RNA sequence called the spliced leader (SL) is trans-spliced from the 100-nucleotide-long SL RNA to some mRNAs. We have identified a trans-spliced leader (SL2) whose sequence differs from that of the original spliced leader (SL1), although both are 22 nucleotides long. By primer-extension sequencing, SL2 but not SL1 was shown to be present at the 5' end of the mRNA encoded by one of the four glyceraldehyde-3-phosphate dehydrogenase genes. The other three glyceraldehyde-3-phosphate dehydrogenase genes encode mRNAs that have the SL1 but not the SL2 sequence at their 5' ends. Therefore, the trans-splicing process can discriminate the transfer of SL1 from that of SL2 in a gene-specific manner.
在线虫秀丽隐杆线虫中，一种名为剪接前导序列（SL）的22个核苷酸的RNA序列从100个核苷酸长的SL RNA反式剪接到一些mRNA上。我们鉴定出了一种反式剪接前导序列（SL2），其序列与原始剪接前导序列（SL1）不同，尽管两者长度均为22个核苷酸。通过引物延伸测序表明，在四个甘油醛-3-磷酸脱氢酶基因之一编码的mRNA的5'端存在SL2而非SL1。其他三个甘油醛-3-磷酸脱氢酶基因编码的mRNA在其5'端具有SL1序列而非SL2序列。因此，反式剪接过程能够以基因特异性的方式区分SL1和SL2的转移。
