编码秀丽隐杆线虫5S核糖体RNA的基因。

Genes coding for 5S ribosomal RNA of the nematode Caenorhabditis elegans.

作者信息

Nelson D W, Honda B M

出版信息

Gene. 1985;38(1-3):245-51. doi: 10.1016/0378-1119(85)90224-0.

Abstract

We have identified a 1-kb genomic sequence that represents the major class of 5S rRNA genes in the nematode Caenorhabditis elegans. This 1-kb sequence is tandemly repeated 110 times in the haploid genome forming a single homogeneous gene family. Other nematode genomic sequences, distinct from the major 1-kb repeat class but homologous to it, may represent dispersed 5S rRNA genes or the ends of a gene cluster. One such fragment shows a restriction fragment length difference between two C. elegans strains. This should allow the genetic analysis of 5S rRNA-coding DNA (5S X rDNA) and its flanking regions in C. elegans.

摘要

我们已经鉴定出一段1千碱基对（kb）的基因组序列，它代表了线虫秀丽隐杆线虫中5S核糖体RNA（rRNA）基因的主要类别。这段1 kb的序列在单倍体基因组中串联重复110次，形成了一个单一的同源基因家族。其他线虫基因组序列，与主要的1 kb重复类别不同但与之同源，可能代表分散的5S rRNA基因或基因簇的末端。其中一个这样的片段在两种秀丽隐杆线虫菌株之间显示出限制性片段长度差异。这应该有助于对秀丽隐杆线虫中5S rRNA编码DNA（5S X rDNA）及其侧翼区域进行遗传分析。