Toropova Yana G, Golovkin Alexey S, Malashicheva Anna B, Korolev Dmitry V, Gorshkov Andrey N, Gareev Kamil G, Afonin Michael V, Galagudza Michael M
Laboratory of Cardioprotection, Institute of Experimental Medicine, Federal Almazov North-West Medical Research Centre, Saint Petersburg, Russian Federation.
Gene and Cell Engineering Group, Institute of Molecular Biology and Genetics, Federal Almazov North-West Medical Research Centre, Saint Petersburg, Russian Federation.
Int J Nanomedicine. 2017 Jan 13;12:593-603. doi: 10.2147/IJN.S122580. eCollection 2017.
Over the last decade, magnetic iron oxide nanoparticles (IONPs) have drawn much attention for their potential biomedical applications. However, serious in vitro and in vivo safety concerns continue to exist. In this study, the effects of uncoated, FeO-SiO composite flake-like, and SiO-FeO core-shell IONPs on cell viability, function, and morphology were tested 48 h postincubation in human umbilical vein endothelial cell culture. Cell viability and apoptosis/necrosis rate were determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and annexin V-phycoerythrin kit, respectively. Cell morphology was evaluated using bright-field microscopy and forward and lateral light scattering profiles obtained with flow cytometry analysis. All tested IONP types were used at three different doses, that is, 0.7, 7.0, and 70.0 μg. Dose-dependent changes in cell morphology, viability, and apoptosis rate were shown. At higher doses, all types of IONPs caused formation of binucleated cells suggesting impaired cytokinesis. FeO-SiO composite flake-like and SiO-FeO core-shell IONPs were characterized by similar profile of cytotoxicity, whereas bare IONPs were shown to be less toxic. The presence of either silica core or silica nanoflakes in composite IONPs can promote cytotoxic effects.
在过去十年中，磁性氧化铁纳米颗粒（IONPs）因其潜在的生物医学应用而备受关注。然而，体外和体内的严重安全问题仍然存在。在本研究中，在人脐静脉内皮细胞培养物中孵育48小时后，测试了未包覆的、FeO-SiO复合片状和SiO-FeO核壳型IONPs对细胞活力、功能和形态的影响。分别使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐法和膜联蛋白V-藻红蛋白试剂盒测定细胞活力和凋亡/坏死率。使用明场显微镜和通过流式细胞术分析获得的前向和侧向光散射谱评估细胞形态。所有测试的IONP类型均以三种不同剂量使用，即0.7、7.0和70.0μg。结果显示细胞形态、活力和凋亡率存在剂量依赖性变化。在较高剂量下，所有类型的IONPs都会导致双核细胞形成，表明胞质分裂受损。FeO-SiO复合片状和SiO-FeO核壳型IONPs具有相似的细胞毒性特征，而裸IONPs的毒性较小。复合IONPs中二氧化硅核或二氧化硅纳米片的存在均可促进细胞毒性作用。
