Department of Pharmaceutical Technology, Bharathidasan Institute of Technology, Anna University, Tiruchirappalli-620024, Tamilnadu, India.
IET Nanobiotechnol. 2020 Dec;14(9):774-784. doi: 10.1049/iet-nbt.2020.0055.
() infection shows a growing burden on human health, and it has become challenging to search for treatment. Therefore, this work focused on the antifungal activity, and cytotoxic effect of biosynthesised nanostructures on human ovarian tetracarcinoma cells PA1 and their corresponding mechanism of cell death. Herein, the authors fabricated advanced biosynthesis of uncoated α-FeO and coated α-FeO nanostructures by using the carbohydrate of . The physicochemical features of nanostructures were characterised by UV-visible, high resolution transmission electron microscopy, Fourier transform infrared spectroscopy, and X-ray diffraction. The antifungal activity of these nanostructures against was studied by the broth dilution method, and examined by 2', 7'-dichlorofluorescein diacetate staining. However, their cytotoxic effects against PA1 cell lines were evaluated by MTT and comet assays. Results indicated characteristic rod-shaped nanostructures, and increasing the average size of α-FeO@ZnO nanocomposite (105.2 nm × 29.1 nm) to five times as compared to α-FeO nanoparticles (20.73nm × 5.25 nm). The surface coating of α-FeO by ZnO has increased its antifungal efficiency against . Moreover, the MTT results revealed that α-FeO@ZnO nanocomposite reduces PA1 cell proliferation due to DNA fragmentation (IC 18.5 μg/ml). Continual advances of green nanotechnology and promising findings of this study are in favour of using the construction of rod-shaped nanostructures for therapeutic applications.
()感染对人类健康造成的负担日益加重，寻找治疗方法具有挑战性。因此，这项工作集中研究了生物合成纳米结构的抗真菌活性和对人卵巢四癌细胞 PA1 的细胞毒性作用及其细胞死亡的相应机制。在这里，作者通过使用碳水化合物来制造未涂层的α-FeO 和涂层的α-FeO 纳米结构的先进生物合成。纳米结构的物理化学特性通过紫外-可见光谱、高分辨率透射电子显微镜、傅里叶变换红外光谱和 X 射线衍射进行了表征。通过肉汤稀释法研究了这些纳米结构对 的抗真菌活性，并通过 2',7'-二氯荧光素二乙酸酯染色进行了检测。然而，通过 MTT 和彗星试验评估了它们对 PA1 细胞系的细胞毒性作用。结果表明具有特征性的棒状纳米结构，并且与α-FeO 纳米颗粒（20.73nm×5.25nm）相比，α-FeO@ZnO 纳米复合材料的平均尺寸增加了五倍（105.2nm×29.1nm）。α-FeO 表面涂覆 ZnO 提高了其对 的抗真菌效率。此外，MTT 结果表明，α-FeO@ZnO 纳米复合材料由于 DNA 片段化（IC 18.5μg/ml）而降低了 PA1 细胞的增殖。绿色纳米技术的不断进步和本研究的有希望的发现有利于使用棒状纳米结构的构建用于治疗应用。
