Al-Ezzy Ali Ibrahim Ali, Abood Walaa Najm
Department of Pathology, Diyala University College of Veterinary Medicine, Baqubah, Iraq.
Department of Microbiology, Diyala University College of Medicine, Baqubah, Iraq.
Eurasian J Med. 2016 Oct;48(3):181-185. doi: 10.5152/eurasianjmed.2016.150187.
This study aimed to determine whether anti-rK39 antibodies were diagnostic markers for visceral leishmaniasis (kala-azar) and to evaluate the correlation between age and gender in disease occurrence in Iraqi patients. In addition, it aimed to evaluate the correlation between thyroid hormones, i.e., thyroid-stimulating hormone (TSH), triiodothyronine (T3), and thyroxine (T4) and anti-rK39 antibodies.
Immunochromatographic technique used for anti-rK39 antibodies detection. Enzyme-linked immunosorbent assay was used for determining the serum TSH, T3, and T4 levels.
One hundred thirty-eight patients with visceral leishmaniasis were included. The mean age was 27.65±11.60 years. Sixty-one patients (44.2%) were males, and their mean age was 29.65±11.10 years. The mean age of females was 26.12±11.89 years. Anti-rK39 antibodies were detected in 11.59% of patients. Anti-rK39 antibodies were equally detected (5.8%) in both genders without a significant difference (p=0.212) or correlation between gender and anti-rK39 antibodies (p=0.623). There was neither a significant difference (p>0.05) nor correlation between gender; age groups according to gender and anti-rK39 antibodies (p>0.05). Both males and females who were positive for anti-rK39 antibodies had normal TSH, T3, and T4 levels. Only one patient who was positive for anti-rK39 antibodies had an elevated T4 level (>12 μg/dL). Neither a significant difference nor correlation was reported among genders; anti-rK39 antibody positivity (p>0.05); and TSH, T3, and T4 levels.
Anti-rK39 antibodies, a daignostic marker for visceral leishmaniasis have no correlation with patients age and gender. Serum TSH and T3 levels were not affected by visceral leishmaniasis. Visceral leishmaniasis causes the increase in serum T4 levels. Thyroid involvement appears to be uncommon in patients who present with visceral leishmaniasis.
本研究旨在确定抗rK39抗体是否为内脏利什曼病（黑热病）的诊断标志物，并评估伊拉克患者疾病发生中年龄与性别的相关性。此外，旨在评估甲状腺激素，即促甲状腺激素（TSH）、三碘甲状腺原氨酸（T3）和甲状腺素（T4）与抗rK39抗体之间的相关性。
采用免疫层析技术检测抗rK39抗体。采用酶联免疫吸附测定法测定血清TSH、T3和T4水平。
纳入138例内脏利什曼病患者。平均年龄为27.65±11.60岁。61例患者（44.2%）为男性，其平均年龄为29.65±11.10岁。女性的平均年龄为26.12±11.89岁。11.59%的患者检测到抗rK39抗体。两性中抗rK39抗体的检测率相同（5.8%），无显著差异（p=0.212），性别与抗rK39抗体之间也无相关性（p=0.623）。性别、按性别划分的年龄组与抗rK39抗体之间既无显著差异（p>0.05）也无相关性（p>0.05）。抗rK39抗体呈阳性的男性和女性的TSH、T3和T4水平均正常。仅1例抗rK39抗体呈阳性的患者T4水平升高（>12μg/dL）。性别、抗rK39抗体阳性（p>0.05）以及TSH、T3和T4水平之间均未报告有显著差异或相关性。
抗rK39抗体作为内脏利什曼病的诊断标志物，与患者的年龄和性别无关。血清TSH和T3水平不受内脏利什曼病影响。内脏利什曼病导致血清T4水平升高。在内脏利什曼病患者中，甲状腺受累似乎并不常见。
