Department of Physics and Astronomy, University of Bologna , viale Berti-Pichat 6/2, 40127 Bologna, Italy.
Interdepartmental Centre "L. Galvani" for Integrated Studies of Bioinformatics, Biophysics and Biocomplexity , via Zamboni 67, 40126 Bologna, Italy.
ACS Appl Mater Interfaces. 2017 Mar 1;9(8):6679-6689. doi: 10.1021/acsami.6b12480. Epub 2017 Feb 15.
PSS is a highly conductive material with good thermal and chemical stability and enhanced biocompatibility that make it suitable for bioengineering applications. The electrical control of the oxidation state of PEDOT:PSS films allows modulation of peculiar physical and chemical properties of the material, such as topography, wettability, and conductivity, and thus offers a possible route for controlling cellular behavior. Through the use of (i) the electrophysiological response of the plasma membrane as a biosensor of the ionic availability; (ii) relative abundance around the cells via X-ray spectroscopy; and (iii) atomic force microscopy to monitor PEDOT:PSS film thickness relative to its oxidation state, we demonstrate that redox processes confer to PEDOT:PSS the property to modify the ionic environment at the film-liquid interface through a "sponge-like" effect on ions. Finally, we show how this property offers the capability to electrically control central cellular properties such as viability, substrate adhesion, and growth, paving the way for novel bioelectronics and biotechnological applications.
PSS 是一种高导电性材料，具有良好的热稳定性和化学稳定性，以及增强的生物相容性，使其适用于生物工程应用。PEDOT:PSS 薄膜氧化态的电控制允许对材料的特殊物理和化学性质进行调制，例如形貌、润湿性和导电性，从而为控制细胞行为提供了一种可能的途径。通过使用 (i) 等离子体膜的电生理反应作为离子可用性的生物传感器；(ii) 通过 X 射线光谱测量细胞周围的相对丰度；以及 (iii) 原子力显微镜监测 PEDOT:PSS 薄膜厚度与其氧化态的关系，我们证明氧化还原过程赋予 PEDOT:PSS 通过对离子的“海绵状”效应来改变薄膜-液体界面处离子环境的性质。最后，我们展示了这种性质如何提供电控制细胞活力、基质粘附和生长等核心细胞特性的能力，为新型生物电子学和生物技术应用铺平了道路。
