细胞角蛋白-14促进涎腺腺样囊性癌的集体侵袭。
Cytokeratin-14 contributes to collective invasion of salivary adenoid cystic carcinoma.
作者信息
Gao Xiao-Lei, Wu Jia-Shun, Cao Min-Xin, Gao Shi-Yu, Cen Xiao, Jiang Ya-Ping, Wang Sha-Sha, Tang Ya-Jie, Chen Qian-Ming, Liang Xin-Hua, Tang Yaling
机构信息
State Key Laboratory of Oral Diseases West China Hospital of Stomatology, Sichuan University, Chengdu, People's Republic of China.
Key Laboratory of Fermentation Engineering, Ministry of Education, Hubei University of Technology, Wuhan, People's Republic of China.
出版信息
PLoS One. 2017 Feb 2;12(2):e0171341. doi: 10.1371/journal.pone.0171341. eCollection 2017.
Collective invasion of cells plays a fundamental role in tissue growth, wound healing, immune response and cancer metastasis. This paper aimed to investigate cytokeratin-14 (CK14) expression and analyze its association with collective invasion in the invasive front of salivary adenoid cystic carcinoma (SACC) to uncover the role of collective invasion in SACC. Here, in the clinical data of 121 patients with SACC, the positive expression of CK14 was observed in 35/121(28.93%) of the invasive front of SACC. CK14 expression in the invasive front, local regional recurrence and distant metastasis were independent and significant prognostic factors in SACC patients. Then, we found that in an ex vivo 3D culture assay, CK14 siRNA receded the collective invasion, and in 2D monolayer culture, CK14 overexpression induced a collective SACC cell migration. These data indicated that the presence of characterized CK14+ cells in the invasive front of SACC promoted collective cell invasion of SACC and may be a biomarker of SACC with a worse prognosis.
细胞的集体侵袭在组织生长、伤口愈合、免疫反应和癌症转移中起着至关重要的作用。本文旨在研究细胞角蛋白-14（CK14）的表达，并分析其与涎腺腺样囊性癌（SACC）侵袭前沿集体侵袭的相关性，以揭示集体侵袭在SACC中的作用。在此，在121例SACC患者的临床资料中，在SACC侵袭前沿的35/121（28.93%）观察到CK14阳性表达。CK14在侵袭前沿的表达、局部区域复发和远处转移是SACC患者独立且显著的预后因素。然后，我们发现在体外3D培养试验中，CK14 siRNA减少了集体侵袭，而在2D单层培养中，CK14过表达诱导了SACC细胞的集体迁移。这些数据表明，SACC侵袭前沿特征性CK14+细胞的存在促进了SACC细胞的集体侵袭，可能是预后较差的SACC的一个生物标志物。
Zotero 插件