Antioxidant activity, phenolic-flavonoid content and high-performance liquid chromatography profiling of three different variants of seeds: A comparative study.

BACKGROUND

The medicinally important phytochemicals present in seeds probably accounts for its wide use in traditional systems of medicines in India, like Ayurveda, Unani, and Siddha.

AIM

The aim of the study was to determine the antioxidant potential of three different geographical variants of seeds and to compare the phenolic profiling to know the effect of geographical variation in phenolic composition.

MATERIALS AND METHODS

Total phenolic and flavonoid content of seeds were analyzed. Antioxidant activities in terms of 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), nitric oxide and superoxide radical scavenging assays were performed. The most active fractions were subjected to high-performance liquid chromatography (HPLC) profiling to identify the phenolic composition.

RESULTS

Among all the fractions, 70% methanol fraction of seed showed significant antioxidant potential. There existed a linear correlation between phenolic content and antioxidant activity. HPLC profiling of 70% methanol (ME) fractions of all the variants revealed the presence of phenolic compounds with high concentrations of ellagic acid and gallic acid. The differences in phenolic concentration due to geographical changes might be the reason for higher antioxidant potential showed by 70% ME of Trivandrum variant.

CONCLUSION

70% methanolic fraction of can act as a novel source of natural antioxidant.