Aprotosoaie Ana Clara, Zavastin Daniela Elena, Mihai Cosmin-Teodor, Voichita Gabriela, Gherghel Daniela, Silion Mihaela, Trifan Adriana, Miron Anca
Faculty of Pharmacy, Grigore T. Popa University of Medicine and Pharmacy Iasi, Universitatii Str. 16, 700115 Iasi, Romania.
Interdisciplinary Research Department-Field Science, Alexandru Ioan Cuza University of Iasi, Carol I Bd. 20A, 700506 Iasi, Romania; National Institute of Research and Development for Biological Sciences/Biological Research Institute, Lascar Catargi Str. 47, 700107 Iasi, Romania.
Food Chem Toxicol. 2017 Oct;108(Pt B):429-437. doi: 10.1016/j.fct.2017.02.006. Epub 2017 Feb 5.
Ramaria largentii Marr & D. E. Stuntz (orange coral mushroom) is a wild edible mushroom whose chemical composition and bioactivity have not been investigated. Herein, we present a study on the phenolic constituents, antioxidant and antigenotoxic effects of a hydromethanolic extract of the fruiting bodies. Total phenolic content, estimated by Folin-Ciocalteu assay, was found to be 42.33 ± 0.18 mg GAE/g. Protocatechuic and vanillic acids were detected by HPLC-DAD-ESI-MS. The extract showed good free radical scavenging and reducing capacities (EC = 64.3 ± 0.2 and 61.54 ± 0.46 μg/mL, respectively). In normal Vero cells, the extract (100, 200 and 300 μg/mL) showed no genotoxic potential and moreover, almost completely protected DNA against HO-induced damage (2.09-7.91% tail DNA) (24 and 48 h pre-treatment). Taken together, the results of our study show that Ramaria largentii extract is devoid of genotoxicity and has a remarkable DNA protective activity against HO-induced damage.
大银枝瑚菌（Ramaria largentii Marr & D. E. Stuntz）（橙色珊瑚菌）是一种野生可食用蘑菇，其化学成分和生物活性尚未得到研究。在此，我们展示了一项关于子实体水甲醇提取物的酚类成分、抗氧化和抗遗传毒性作用的研究。通过福林-西奥尔特法（Folin-Ciocalteu assay）估计，总酚含量为42.33±0.18毫克没食子酸当量/克。原儿茶酸和香草酸通过高效液相色谱-二极管阵列-电喷雾电离质谱法（HPLC-DAD-ESI-MS）检测。该提取物显示出良好的自由基清除和还原能力（EC分别为64.3±0.2和61.54±0.46微克/毫升）。在正常的非洲绿猴肾细胞（Vero cells）中，该提取物（100、200和300微克/毫升）没有显示出遗传毒性潜力，而且，几乎完全保护DNA免受羟基自由基（HO）诱导的损伤（尾部DNA为2.09 - 7.91%）（预处理24和48小时）。综上所述，我们的研究结果表明，大银枝瑚菌提取物没有遗传毒性，并且对羟基自由基诱导的损伤具有显著的DNA保护活性。
