Kischer C W, Sheridan D, Pindur J
Department of Anatomy, University of Arizona College of Medicine, Tucson 85724.
Anat Rec. 1989 Nov;225(3):189-96. doi: 10.1002/ar.1092250303.
Hypertrophic scars and keloids appear to be unique to humans since animals are not known to form these lesions. Therefore, in an effort to develop an experimental model for their study, implants of these human lesions were made in nude (athymic) mice (nu/nu) in suprascapular subcutaneous pockets. The implants were recovered from 2 to 246 days. By histological and fine structural parameters all implants remained viable and their morphological character was maintained. Selected mice were injected with barium to confirm by microangiography vascular flow between mouse and implant. Hoechst stain for DNA, used to distinguish mouse cells from human cells, confirmed vascular anastamosis between host and implant: barium-filled vessels in the interior of the implant demonstrated human endothelial cells. Peripheral vascularization of the implant with minimal ingrowth of mouse vessels occurs during the first 8 days. Anastamosis probably occurs sometime before 16 days postimplantation, or earlier, depending upon the availability of patent microvessels in the implanted tissue. The presence of the implant does not appear to prompt a continuing vascular growth into or throughout the implant. The time frame of 16 days postimplantation should be taken into account when developing schemata of experimental or therapeutic modalities.
肥厚性瘢痕和瘢痕疙瘩似乎是人类所特有的，因为目前已知动物不会形成这些损伤。因此，为了开发用于研究它们的实验模型，将这些人类损伤组织植入裸（无胸腺）小鼠（nu/nu）肩胛上皮下囊袋中。植入物在2至246天内取出。通过组织学和精细结构参数评估，所有植入物均保持存活，其形态特征得以维持。给选定的小鼠注射钡剂，通过微血管造影确认小鼠与植入物之间的血管血流。用Hoechst DNA染色来区分小鼠细胞和人类细胞，证实宿主与植入物之间存在血管吻合：植入物内部充满钡剂的血管显示有人类内皮细胞。在最初的8天内，植入物的外周血管化过程中，小鼠血管向内生长极少。血管吻合可能在植入后16天之前的某个时间发生，或者更早，这取决于植入组织中开放微血管的情况。植入物的存在似乎不会促使血管持续长入或贯穿植入物。在制定实验或治疗方案时，应考虑植入后16天这个时间框架。
