Chen Shixuan, Ge Liangpeng, Mueller Aubrey, Carlson Mark A, Teusink Matthew J, Shuler Franklin D, Xie Jingwei
Department of Surgery-Transplant and Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE, United States.
Chongqing Academy of Animal Sciences and Key Laboratory of Pig Industry Sciences, Ministry of Agriculture, Chongqing, China.
Nanomedicine. 2017 May;13(4):1435-1445. doi: 10.1016/j.nano.2017.01.016. Epub 2017 Feb 6.
Surgical site infections (SSIs) represent the most common nosocomial infection among surgical patients. In order to prevent SSIs in a sustained manner and lessen side effects, we developed a twisting method for generation of nanofiber-based sutures capable of simultaneous delivery of silver and gentamicin. The prepared sutures are composed of core-sheath nanofibers with gentamicin/pluronic F127 in the core and silver/PCL in the sheath produced by co-axial electrospinning. The diameters of obtained sutures range from ~80 μm to ~1.2 mm. The in vitro release profiles of silver and gentamicin exhibit an initial burst followed by a sustained release over 5 weeks. The co-encapsulated sutures were able to kill bacteria much more effectively than gentamicin or silver alone loaded nanofiber sutures, without showing obvious impact on proliferation and migration of dermal fibroblasts and keratinocytes. The gentamicin and silver co-loaded PCL nanofiber sutures may hold great potential for prevention of SSIs.
手术部位感染（SSIs）是外科手术患者中最常见的医院感染。为了持续预防手术部位感染并减轻副作用，我们开发了一种用于制备基于纳米纤维缝线的捻合方法，该缝线能够同时递送银和庆大霉素。所制备的缝线由核壳纳米纤维组成，其核心为庆大霉素/普朗尼克F127，外壳为银/聚己内酯，通过同轴静电纺丝制备而成。所获得缝线的直径范围为80μm至1.2mm。银和庆大霉素的体外释放曲线显示出初始的突释，随后在5周内持续释放。与单独负载庆大霉素或银的纳米纤维缝线相比，共包封的缝线能够更有效地杀灭细菌，且对真皮成纤维细胞和角质形成细胞的增殖和迁移没有明显影响。负载庆大霉素和银的聚己内酯纳米纤维缝线在预防手术部位感染方面可能具有巨大潜力。
