Garjani A, Tila D, Hamedeyazdan S, Vaez H, Rameshrad M, Pashaii M, Fathiazad F
Department of Pharmacognosy, School of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran, Iran, Islamic Republic Of.
Folia Morphol (Warsz). 2017;76(3):361-371. doi: 10.5603/FM.a2017.0011. Epub 2017 Feb 15.
The aim of this study was to evaluate the cardioprotective effects of aqueous fraction of Marrubium vulgare hydroalcoholic extract on cardiac parameters in ischaemic-reperfused isolated rat hearts.
The aerial parts of the plant were extracted with methanol 70% by maceration. The water-soluble portion of the total hydroalcoholic extract was prepared with liquid-liquid extraction (LLE). Afterwards, the antioxidant activity, total phenolic and flavonoids content of the aqueous fraction were determined. In order to evaluate the effects of the aqueous fraction on cardiac parameters and ischaemia-reperfusion (I/R) injury, the Langendroff method was used on male Wistar rats. Harvested hearts were cannulated immediately to the Langendroff apparatus and subjected into 30 min regional ischaemia and 2 h reperfusion, either by a modified Krebs-Henseleit buffer (KHB) solution or enriched KHB solution with plant extract (10, 20, 40 μg/mL).
The aqueous fraction was found to be a scavenger of DPPH radical with RC50 value of 47 μg/mL. The total phenolic and flavonoids content of the fraction was 6.05 g gallic acid equivalent and 36.13 mg quercetin equivalent per 100 g of dry plant material. In addition, 40 μg/mL of Marrubium vulgare aqueous fraction significantly decreased infarct size in comparison to control group. All doses considerably reduced the total ventricular ectopic beats during 30 min of ischaemia. The extract at dose of 40 μg/mL noticeably decreased the arrhythmias during the first 30 min of reperfusion.
The results of the study indicated aqueous fraction of Marrubium vulgare possesses a protective effect against I/R injuries in isolated rat hearts.
本研究旨在评估夏至草水醇提取物的水相部分对缺血再灌注离体大鼠心脏心脏参数的心脏保护作用。
采用浸渍法用70%甲醇提取该植物地上部分。通过液液萃取（LLE）制备总水醇提取物的水溶性部分。之后，测定水相部分的抗氧化活性、总酚和黄酮含量。为了评估水相部分对心脏参数和缺血再灌注（I/R）损伤的影响，对雄性Wistar大鼠采用Langendroff方法。收获的心脏立即插管到Langendroff装置中，并通过改良的Krebs-Henseleit缓冲液（KHB）溶液或添加植物提取物（10、20、40μg/mL）的富集KHB溶液进行30分钟的局部缺血和2小时的再灌注。
发现水相部分是DPPH自由基清除剂，RC50值为47μg/mL。每100克干燥植物材料中该部分的总酚和黄酮含量分别为6.05克没食子酸当量和36.13毫克槲皮素当量。此外，与对照组相比，40μg/mL的夏至草水相部分显著降低了梗死面积。所有剂量均显著减少了缺血30分钟期间的总室性早搏。40μg/mL剂量的提取物在再灌注的前30分钟显著降低了心律失常。
研究结果表明，夏至草的水相部分对离体大鼠心脏的I/R损伤具有保护作用。
