Yao Huan-Yin, Liu Wei-Rong, Zhang Hang-Hu, Li Hua-Jun, Wang Xiao-Xian, Liu Shu-Mei, Chen Xiao-Hong
Department of Pediatrics, Shaoxing People's Hospital of Zhejiang Province/Shaoxing Hospital of Zhejiang University, Shaoxing, Zhejiang 312000, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2017 Feb;19(2):163-166. doi: 10.7499/j.issn.1008-8830.2017.02.007.
To investigate the effect of atopy on the expression of glucocorticoid receptors in children with bronchiolitis.
ELISA was used to measure the changes in the serum levels of glucocorticoid receptor α (GRα) and glucocorticoid receptor β (GRβ) in the bronchiolitis group (77 children, including 34 children with atopy) and pneumonia group (68 children). Thirty-eight children who were prepared to undergo surgeries for non-infectious diseases and had no atopy or family history of allergic diseases were enrolled as the control group.
The bronchiolitis group and the pneumonia group had significant increases in the serum levels of GRα and GRβ compared with the control group (P<0.01), and the bronchiolitis group had significant increases in these levels compared with the pneumonia group (P<0.01). Compared with the control group and the pneumonia group, the bronchiolitis group had a significant increase in the GRα/GRβ ratio (P<0.01). Compared with the control group, the children with or without atopy in the bronchiolitis group had significant increases in the serum levels of GRα and GRβ (P<0.01). The non-atopic children in the bronchiolitis group had a significant increase in the serum level of GRβ compared with the atopic children (P<0.01). The atopic children in the bronchiolitis group had a significant increase in the GRα/GRβ ratio compared with the control group and non-atopic children in the bronchiolitis group (P<0.01).
Children with bronchiolitis have increased serum levels of GRα and GRβ. The children with atopy have an increased GRα/GRβ ratio, suggesting that the atopic children with bronchiolitis are highly sensitive to glucocorticoids.
探讨特应性对细支气管炎患儿糖皮质激素受体表达的影响。
采用酶联免疫吸附测定法（ELISA）检测细支气管炎组（77例患儿，其中34例有特应性）、肺炎组（68例患儿）血清糖皮质激素受体α（GRα）和糖皮质激素受体β（GRβ）水平的变化。选取38例准备接受非感染性疾病手术且无特应性或过敏性疾病家族史的患儿作为对照组。
与对照组相比，细支气管炎组和肺炎组血清GRα和GRβ水平显著升高（P<0.01），且细支气管炎组这些水平高于肺炎组（P<0.01）。与对照组和肺炎组相比，细支气管炎组GRα/GRβ比值显著升高（P<0.01）。与对照组相比，细支气管炎组有或无特应性的患儿血清GRα和GRβ水平均显著升高（P<0.01）。细支气管炎组中非特应性患儿血清GRβ水平高于特应性患儿（P<0.01）。细支气管炎组中特应性患儿的GRα/GRβ比值高于对照组和细支气管炎组中的非特应性患儿（P<0.01）。
细支气管炎患儿血清GRα和GRβ水平升高。有特应性的患儿GRα/GRβ比值升高，提示细支气管炎伴特应性的患儿对糖皮质激素高度敏感。
