Hajiluian Ghazaleh, Abbasalizad Farhangi Mahdieh, Jahangiry Leila
Student Research Committee, Nutrition Research Center, Faculty of Nutrition, Tabriz University of Medical Sciences, Tabriz, Iran.
Nutrition Research Center, Drug Applied Research Center, Department of Community Nutrition, Tabriz University of Medical Sciences, Tabriz, Iran.
PLoS One. 2017 Feb 17;12(2):e0171637. doi: 10.1371/journal.pone.0171637. eCollection 2017.
To evaluate the relationship between Mediterranean dietary pattern, anthropometric and metabolic biomarkers and vascular endothelial growth factor (VEGF) +405 G/C gene polymorphism in patient with metabolic syndrome (Mets).
In this study 150 patients with Mets and 50 healthy subjects were enrolled. Dietary intakes were evaluated with a semi-quantitative food-frequency questionnaire (FFQ) and Mediterranean dietary quality index (Med-DQI) was assessed. Anthropometric assessments and blood pressure measurement were performed. Biochemical assays including fasting serum glucose (FSG), matrix metalloproteinase-3 (MMP-3), liver enzymes and lipid profiles were also assessed. Polymorphism of +405 G/C VEGF gene was determined utilizing polymerase chain reaction-restriction fragments length polymorphism (PCR-RFLP) method.
Serum high density lipoprotein-cholesterol (HDL-C) was significantly lower and low density lipoprotein cholesterol (LDL-C), triglyceride (TG), total cholesterol (TC) concentrations and FSG were significantly higher in metabolic syndrome patients compared with control group (P < 0.05). Metabolic syndrome group with high consumption of "cholesterol" had significantly upper serum TG; also high consumption of "fish" and "vegetables-fruits" was associated with a significantly lower serum LDL concentrations. In metabolic syndrome patients with CC genotype, mean score of "saturated fatty acid" subgroup was significantly higher compared with other genotypes; whereas, in healthy individuals, mean score of "fruit-vegetable" subgroup in individuals of CC and GG genotype was significantly higher (P<0.05).
Our findings indicated a significant relationship between Mediterranean dietary quality index and both anthropometric and metabolic risk factors. We also indicated a higher "saturated fatty acid" intake in CC genotype among metabolic syndrome patients.
评估代谢综合征（Mets）患者地中海饮食模式、人体测量和代谢生物标志物与血管内皮生长因子（VEGF）+405 G/C基因多态性之间的关系。
本研究纳入了150例Mets患者和50例健康受试者。通过半定量食物频率问卷（FFQ）评估饮食摄入量，并评估地中海饮食质量指数（Med-DQI）。进行人体测量评估和血压测量。还评估了包括空腹血糖（FSG）、基质金属蛋白酶-3（MMP-3）、肝酶和血脂谱在内的生化指标。利用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法测定VEGF基因+405 G/C多态性。
与对照组相比，代谢综合征患者的血清高密度脂蛋白胆固醇（HDL-C）显著降低，低密度脂蛋白胆固醇（LDL-C）、甘油三酯（TG）、总胆固醇（TC）浓度和FSG显著升高（P<0.0
