Somosy Z, Kubasova T, Köteles G J
Frédéric Joliot-Curie, National Research Institute for Radiobiology and Radiohygiene, Budapest, Hungary.
Scanning Microsc. 1987 Sep;1(3):1267-78.
The cellular membrane as one of the targets of ionizing radiation might play an important role in the development and modification of radiation-induced alterations after low doses. The present paper reviews the micromorphological and functional changes of plasma membranes of irradiated blood and cultured cells with special emphasis on the surface conditions: lectin binding, negative surface charges. The review is completed by our own studies on distribution of positive surface charges and the bindings of two lectins, the Concanavalin A and the wheat germ agglutinin. It was found that the decrease of negative surface charges is unconcomitant with appearance of domains exposing positive ones, particularly on the surfaces of rufflings. The distribution of Concanavalin A binding sites turned from a uniform distribution to a polarized one, especially on apical regions where it appeared in large aggregates. The polarity in localization of wheat germ agglutinin on untreated fibroblasts observed in our experiments ceased shortly after irradiation.
细胞膜作为电离辐射的靶点之一，可能在低剂量辐射诱导的改变的发生和改变过程中起重要作用。本文综述了受辐照血液和培养细胞的质膜的微观形态和功能变化，特别强调了表面状况：凝集素结合、表面负电荷。本文还完成了我们自己关于表面正电荷分布以及两种凝集素（伴刀豆球蛋白A和麦胚凝集素）结合情况的研究。研究发现，表面负电荷的减少与暴露正电荷区域的出现并非同时发生，特别是在褶皱表面。伴刀豆球蛋白A结合位点的分布从均匀分布变为极化分布，尤其是在顶端区域，在那里它以大聚集体形式出现。在我们的实验中观察到，未处理的成纤维细胞上麦胚凝集素定位的极性在辐照后不久就消失了。
