Li Yan, Wang Chunlan, Wang Fangfei, Dong Hailing, Guo Shunxing, Yang Junshan, Xiao Peigen
Institute of Medical Plant Development, Chinese Academy of Medicinal Sciences and Peking Union Medical College, Beijing 100193, China.
Zhongguo Zhong Yao Za Zhi. 2010 Jul;35(13):1715-9. doi: 10.4268/cjcmm20101314.
The column chromatography on silica gel, sephadex LH-20 preparative HPLC were used to separate and purify the compounds from the stems of Dendrobium candidum. Twenty compounds were isolated and identified as 3,4'-dihydroxy-5-methoxybibenzyl(1), dihydroresveratrol(2), dendromoniliside E(3), denbinobin(4),2,4,7-trihydroxy-9, 10-dihydrophenanthrene(5), aduncin(6), (-)-loliolide(7), adenosine(8), uridine(9), guanosine(10), sucrose(11), 5-hydroxymethyl-furaldehyde(12), n-octacostyl ferulate(13), defuscin(14), n-triacontyl cis-p-coumarate(15), daucosterol(16), beta-sitosterol(17), hexadecanoic acid(18), hentriacontane(19), and heptadecanoic acid(20). Their structures were elucidated on the basis of spectroscopic data and physicochemical properties. All of the compounds were isolated from this plant for the first time.
采用硅胶柱色谱、葡聚糖凝胶LH - 20柱色谱及制备型高效液相色谱法从铁皮石斛茎中分离纯化化合物。分离鉴定了20个化合物，分别为3,4'-二羟基-5-甲氧基联苄(1)、二氢白藜芦醇(2)、石斛酮酚苷E(3)、石斛醌(4)、2,4,7-三羟基-9,10-二氢菲(5)、钩状石斛素(6)、(-)-环乙酰草酰乙酸内酯(7)、腺苷(8)、尿苷(9)、鸟苷(10)、蔗糖(11)、5-羟甲基糠醛(12)、正二十八烷基阿魏酸酯(13)、德福新(14)、正三十烷基顺式对香豆酸酯(15)、胡萝卜苷(16)、β-谷甾醇(17)、十六烷酸(18)、三十一烷(19)和十七烷酸(20)。通过光谱数据和理化性质对其结构进行了鉴定。所有化合物均为首次从该植物中分离得到。
