假臭草叶乙醇提取物对高脂饮食瘦大鼠的抗肥胖作用。
Anti-obesity effect of ethanolic extract from Cosmos caudatus Kunth leaf in lean rats fed a high fat diet.
作者信息
Rahman Hafeedza Abdul, Sahib Najla Gooda, Saari Nazamid, Abas Faridah, Ismail Amin, Mumtaz Muhammad Waseem, Hamid Azizah Abdul
机构信息
School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, UKM Bangi, Selangor, Malaysia.
Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia.
出版信息
BMC Complement Altern Med. 2017 Feb 22;17(1):122. doi: 10.1186/s12906-017-1640-4.
BACKGROUND
Obesity is a major health concern both in developed and developing countries. The use of herbal medicines became the subject of interest for the management of obesity due to its natural origin, cost effectiveness and minimal side effects. The present study aimed at investigating anti-obesity potential of ethanolic extract from Cosmos caudatus Kunth leaf (EECCL).
METHODS
In this study, the rats were randomly divided into six groups i.e., (1) Normal Diet (ND); (2) Normal Diet and 175 mg/kgBW of EECCL (ND + 175 mg/kgBW); (3) Normal Diet and 350 mg/kgBW of EECCL (ND + 350 mg/kgBW); (4) High Fat Diet (HFD); (5) High Fat Diet and 175 mg/kgBW of EECCL (HFD + 175 mg/kgBW); (6) High Fat Diet and 350 mg/kgBW of EECCL (HFD + 350 mg/kgBW). The anti-obesity potential was evaluated through analyses of changes in body weight, visceral fat weight, and blood biochemicals including total cholesterol, triglycerides, high-density lipoprotein cholesterol (HDL-c), low-density lipoprotein cholesterol (LDL-c), leptin, insulin, adiponectin, ghrelin and fecal fat content. In addition, metabolite profiling of EECCL was carried out using NMR spectroscopy.
RESULTS
Rats receiving EECCL together with HFD showed significant (p < 0.05) reduction in body weight gain compared to rats receiving HFD only. At the end of study, the body weight gain of EECCL treated rats was not significantly (p > 0.05) different with those of ND rats. Other related obesity biomarkers including plasma lipid profiles, insulin, leptin, ghrelin and adiponectin levels also showed significant improvement (p < 0.05). Administration of EECCL caused significant (p < 0.05) increase in fecal fat excretion, which validates the hypothesis of lipase inhibition, an anti-obesity mechanism similar to standard drug of Orlistat. The H-NMR spectra of EECCL ascertained the presence of catechin, quercetin, rutin, kaempherol and chlorogenic acid in the extract.
CONCLUSION
Conclusively, EECCL showed anti-obesity properties by inhibition of intestinal lipid absorption and modulation of adipocytes markers.
背景
肥胖是发达国家和发展中国家主要的健康问题。由于草药具有天然来源、成本效益高和副作用小的特点，其在肥胖管理中的应用成为人们关注的焦点。本研究旨在探究大花咸丰草叶乙醇提取物（EECCL）的抗肥胖潜力。
方法
在本研究中，大鼠被随机分为六组，即：（1）正常饮食组（ND）；（2）正常饮食并给予175毫克/千克体重的EECCL组（ND + 175毫克/千克体重）；（3）正常饮食并给予350毫克/千克体重的EECCL组（ND + 350毫克/千克体重）；（4）高脂饮食组（HFD）；（5）高脂饮食并给予175毫克/千克体重的EECCL组（HFD + 175毫克/千克体重）；（6）高脂饮食并给予350毫克/千克体重的EECCL组（HFD + 350毫克/千克体重）。通过分析体重、内脏脂肪重量以及血液生化指标（包括总胆固醇、甘油三酯、高密度脂蛋白胆固醇（HDL-c）、低密度脂蛋白胆固醇（LDL-c）、瘦素、胰岛素、脂联素、胃泌素和粪便脂肪含量）的变化来评估抗肥胖潜力。此外，使用核磁共振光谱对EECCL进行代谢物谱分析。
结果
与仅接受高脂饮食的大鼠相比，接受高脂饮食并同时给予EECCL的大鼠体重增加显著降低（p < 0.05）。在研究结束时，接受EECCL治疗的大鼠体重增加与正常饮食组大鼠相比无显著差异（p > 0.05）。其他与肥胖相关的生物标志物，包括血脂谱、胰岛素、瘦素、胃泌素和脂联素水平也有显著改善（p < 0.05）。给予EECCL导致粪便脂肪排泄显著增加（p < 0.05），这证实了脂肪酶抑制的假设，这是一种类似于标准药物奥利司他的抗肥胖机制。EECCL的氢核磁共振光谱确定提取物中存在儿茶素、槲皮素、芦丁、山奈酚和绿原酸。
结论
总之，EECCL通过抑制肠道脂质吸收和调节脂肪细胞标志物显示出抗肥胖特性。
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