Samiei Fatemeh, Jamshidzadeh Akram, Noorafshan Ali, Ghaderi Amir
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Fars, Iran.
Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Fars, Iran.
Iran J Pharm Res. 2016 Winter;15(Suppl):75-82.
Amiodarone is used in treatment of cardiac arrhythmias. Therapeutic use of amiodarone is limited by its side effects, including pulmonary toxicity. Human Placenta Extract (HPE) contains a variety of bio-active substances. Thus, the present study aimed to quantitatively evaluate the protective effects of HPE on the structural lung changes induced by amiodarone using stereological methods. Sprague-Dawley male rats were divided into four groups. The first, second, and third groups received no treatment, amiodarone (100 mg/kg, i.p.), and HPE (500 µL/kg, i.p.), respectively. The fourth group was treated with amiodarone + HPE. The animals' lungs were removed after 10 days. The lung volume was estimated using the Cavalieri principle on the embedded and cut tissue and corrected for shrinkage. The volume density of the parenchyma, alveolar space, and septa were estimated using point-counting method. The surface area of the alveoli, the volume-weighted means alveoli volume, and mean septum thickness were also estimated in all groups. The total volume and thickness of the alveolar septum were increased by 40 % and 28 %, respectively. However, the total volume of the alveolar space was decreased by 31 % in the amiodarone treated-rats. The mean alveolar volume was decreased by 64 % on the average in the amiodarone treated group. Yet, these changes were not detected in the amiodarone+HPE group. Moreover, RBC accumulation in the alveolar space and septa was ameliorated after HPE treatment. HPE can protect the lung tissue from the structural changes induced by amiodarone.
胺碘酮用于治疗心律失常。胺碘酮的治疗应用因其副作用（包括肺毒性）而受到限制。人胎盘提取物（HPE）含有多种生物活性物质。因此，本研究旨在使用体视学方法定量评估HPE对胺碘酮诱导的肺部结构变化的保护作用。将Sprague-Dawley雄性大鼠分为四组。第一组、第二组和第三组分别不接受治疗、接受胺碘酮（100 mg/kg，腹腔注射）和HPE（500 μL/kg，腹腔注射）。第四组用胺碘酮+HPE治疗。10天后取出动物的肺。使用卡瓦列里原理对包埋和切片的组织估计肺体积，并对收缩进行校正。使用点计数法估计实质、肺泡腔和间隔的体积密度。还估计了所有组的肺泡表面积、体积加权平均肺泡体积和平均间隔厚度。肺泡间隔的总体积和厚度分别增加了40%和28%。然而，在接受胺碘酮治疗的大鼠中，肺泡腔的总体积减少了31%。在接受胺碘酮治疗的组中，平均肺泡体积平均减少了64%。然而，在胺碘酮+HPE组中未检测到这些变化。此外，HPE治疗后肺泡腔和间隔中的红细胞积聚得到改善。HPE可以保护肺组织免受胺碘酮诱导的结构变化的影响。
