Moreno-Lopez J, Ahola H, Eriksson A, Bergman P, Pettersson U
Department of Veterinary Microbiology, Biomedical Center, Uppsala, Sweden.
J Virol. 1987 Nov;61(11):3394-400. doi: 10.1128/JVI.61.11.3394-3400.1987.
A papillomavirus was isolated from the epithelial layer of a cutaneous fibropapilloma on a Swedish reindeer (Rangifer tarandus). Reindeer papillomavirus (RPV) is morphologically indistinguishable from other papillomaviruses, but the restriction enzyme cleavage pattern of its genome is different. No sequence homology was detected between RPV DNA and the DNAs of bovine papillomavirus type 1 (BPV-1) and avian papillomavirus when hybridization was performed under stringent conditions. However, the RPV genome hybridized to the genome of the European elk papillomavirus and the deer papillomavirus under stringent conditions. A physical map of the RPV genome was constructed, and selected regions of the genome, covering the open translational reading frame (ORF) E5 and part of the E1 and L1 ORFs, were studied by nucleotide sequence analysis. The results made it possible to align the RPV genome with the genome of BPV-1. The E5 ORF of RPV has the potential to encode a 44-amino-acid, exceptionally hydrophobic polypeptide which is very similar to the E5 polypeptides of BPV-1 and deer and European elk papillomaviruses. RPV is oncogenic for hamsters and transforms C127 mouse cells in vitro. Several virus-specific mRNAs were detected in RPV-transformed C127 cells.
从一只瑞典驯鹿（Rangifer tarandus）皮肤纤维乳头瘤的上皮层中分离出一种乳头瘤病毒。驯鹿乳头瘤病毒（RPV）在形态上与其他乳头瘤病毒无法区分，但其基因组的限制性内切酶切割模式不同。当在严格条件下进行杂交时，未检测到RPV DNA与1型牛乳头瘤病毒（BPV-1）和禽乳头瘤病毒的DNA之间存在序列同源性。然而，在严格条件下，RPV基因组与欧洲驼鹿乳头瘤病毒和鹿乳头瘤病毒的基因组发生了杂交。构建了RPV基因组的物理图谱，并通过核苷酸序列分析研究了基因组的选定区域，这些区域覆盖开放翻译阅读框（ORF）E5以及E1和L1 ORF的部分区域。结果使得能够将RPV基因组与BPV-1的基因组进行比对。RPV的E5 ORF有潜力编码一种44个氨基酸的、异常疏水的多肽，该多肽与BPV-1以及鹿和欧洲驼鹿乳头瘤病毒的E5多肽非常相似。RPV对仓鼠具有致癌性，并能在体外转化C127小鼠细胞。在RPV转化的C127细胞中检测到了几种病毒特异性mRNA。
