Ahola H, Bergman P, Ström A C, Moreno-Lopéz J, Pettersson U
Gene. 1986;50(1-3):195-205. doi: 10.1016/0378-1119(86)90324-0.
The nucleotide sequence of the early (transforming) region from the European elk papillomavirus (EEPV) double-stranded DNA has been determined together with flanking regions. The established sequence, which is 5732 bp long, shows that the genome of EEPV is closely related to the previously sequenced bovine papillomavirus type 1 (BPV-1) and deer papillomavirus (DPV) genomes. Seven open reading frames (ORFs), designated E1-E7, were identified in similar positions as in the BPV-1 genome. The E1 and E5 regions were best conserved. The strong homology between the E5 ORFs of EEPV, BPV-1 and DPV is interesting in the light of the recent proposal that these ORFs encode a major transforming function (Schiller et al., 1986; DiMaio et al., 1986). A set of mRNAs, comprising six size classes, were identified in EEPV-transformed cells. At least two different promoters appear to control EEPV transcription in transformed cells.
已确定欧洲驼鹿乳头瘤病毒（EEPV）双链DNA早期（转化）区域的核苷酸序列及其侧翼区域。确定的序列长度为5732 bp，表明EEPV的基因组与先前测序的1型牛乳头瘤病毒（BPV-1）和鹿乳头瘤病毒（DPV）基因组密切相关。在与BPV-1基因组相似的位置鉴定出七个开放阅读框（ORF），命名为E1-E7。E1和E5区域保守性最好。鉴于最近有人提出这些ORF编码主要转化功能（席勒等人，1986年；迪马约等人，1986年），EEPV、BPV-1和DPV的E5 ORF之间的高度同源性很有意思。在EEPV转化的细胞中鉴定出一组由六个大小类别组成的mRNA。至少有两个不同的启动子似乎控制着转化细胞中EEPV的转录。
