Institute of Entomology, Biology Centre CAS, Branisovska 31, 370 05 Ceske Budejovice, Czech Republic.
Faculty of Science, University of South Bohemia, Branisovska 31, 370 05 Ceske Budejovice, Czech Republic.
Sci Rep. 2017 Feb 23;7:43273. doi: 10.1038/srep43273.
Drosophila imaginal disc growth factor 2 (IDGF2) is a member of chitinase-like protein family (CLPs) able to induce the proliferation of imaginal disc cells in vitro. In this study we characterized physiological concentrations and expression of IDGF2 in vivo as well as its impact on the viability and transcriptional profile of Drosophila cells in vitro. We show that IDGF2 is independent of insulin and protects cells from death caused by serum deprivation, toxicity of xenobiotics or high concentrations of extracellular adenosine (Ado) and deoxyadenosine (dAdo). Transcriptional profiling suggested that such cytoprotection is connected with the induction of genes involved in energy metabolism, detoxification and innate immunity. We also show that IDGF2 is an abundant haemolymph component, which is further induced by injury in larval stages. The highest IDGF2 accumulation was found at garland and pericardial nephrocytes supporting its role in organismal defence and detoxification. Our findings provide evidence that IDGF2 is an important trophic factor promoting cellular and organismal survival.
果蝇胚盘生长因子 2（IDGF2）是几丁质酶样蛋白家族（CLPs）的成员，能够在体外诱导胚盘细胞的增殖。在这项研究中，我们描述了 IDGF2 在体内的生理浓度和表达，以及它对果蝇细胞体外活力和转录谱的影响。我们表明，IDGF2 不依赖胰岛素，并能保护细胞免受血清剥夺、外源性毒物、高浓度细胞外腺苷（Ado）和脱氧腺苷（dAdo）引起的死亡。转录谱分析表明，这种细胞保护与诱导参与能量代谢、解毒和先天免疫的基因有关。我们还表明，IDGF2 是一种丰富的血淋巴成分，在幼虫阶段受到损伤后进一步诱导。IDGF2 的最高积累发生在花环和围心肾细胞中，支持它在机体防御和解毒中的作用。我们的发现提供了证据表明，IDGF2 是一种重要的营养因子，促进细胞和机体的存活。
