Borysowski Jan, Międzybrodzki Ryszard, Wierzbicki Piotr, Kłosowska Danuta, Korczak-Kowalska Grażyna, Weber-Dąbrowska Beata, Górski Andrzej
Department of Clinical Immunology, Transplantation Institute, Medical University of Warsaw, Nowogrodzka Str. 59, 02-006 Warsaw, Poland.
Laboratory of Bacteriophages, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, RudolfaWeigla Str. 12, 53-114 Wrocław, Poland.
Viruses. 2017 Feb 21;9(2):36. doi: 10.3390/v9020036.
The objective of this study was to evaluate the effects of A3R phage and lysate obtained after phage infection on neutrophil degranulation. The exocytosis of primary and secondary granules from neutrophils was investigated in vitro in whole blood specimens by flow cytometry based on the expression of specific markers of exocytosis (CD63 for primary granules and CD66b for secondary granules). We found that both A3R and lysate had no significant effect on the exocytosis of primary and secondary granules. These data suggest that neither A3R virions nor any products of phage-induced lysis of are likely to induce neutrophil degranulation in patients who are treated with phage preparations. Since neutrophil granules contain some potentially toxic proteins, our results provide an important argument for the safety of phage therapy. Moreover, these data indicate that the induction of neutrophil degranulation is not likely to contribute to antibacterial effects of phages.
本研究的目的是评估A3R噬菌体以及噬菌体感染后获得的裂解物对中性粒细胞脱颗粒的影响。基于胞吐作用的特异性标志物（初级颗粒为CD63，次级颗粒为CD66b）的表达，通过流式细胞术在全血标本中体外研究了中性粒细胞初级和次级颗粒的胞吐作用。我们发现A3R和裂解物对初级和次级颗粒的胞吐作用均无显著影响。这些数据表明，在接受噬菌体制剂治疗的患者中，A3R病毒粒子以及噬菌体诱导裂解的任何产物都不太可能诱导中性粒细胞脱颗粒。由于中性粒细胞颗粒含有一些潜在的毒性蛋白，我们的结果为噬菌体治疗的安全性提供了重要依据。此外，这些数据表明中性粒细胞脱颗粒的诱导不太可能对噬菌体的抗菌作用有贡献。
