Leishmaniasis involves the participation of several species of both wild and domestic mammal hosts and sandfly vectors, which demonstrates the eco-epidemiological complexity observed in this disease. Bats are among the most abundant types of mammals and the scarcity of research on infection in these animals gives evidence of the importance of new studies that aim to clarify this relationship. This study aimed to detect the spp. in bats. 146 bats, representing 16 different species belonging to the Molossidae, Vespertilionidae, and Phyllostomidae families, were received and processed for collection of tissues. Skin samples were collected from 100% of the bats, and liver samples were collected from 87% (n = 127). After evaluating the quality of the DNA extracted by means of PCR directed to the IRBP gene, the samples considered suitable for the detection test were submitted for PCR directed to kDNA, and to confirm positivity, were tested to the SSUrRNA gene-directed Nested-PCR. The presence in the species , and are the first reports this encounter in these species of bats in Brazil. Furthermore, new species of bats as possible hosts for are reported, such as , and, for , and . These findings in bats in an area endemic for leishmaniasis indicate that these animals may be involved in sustaining the disease cycle in this location.