Geiselbrecht A D, Herwig R P, Deming J W, Staley J T
Department of Microbiology, University of Washington, Seattle 98195, USA.
Appl Environ Microbiol. 1996 Sep;62(9):3344-9. doi: 10.1128/aem.62.9.3344-3349.1996.
Naphthalene- and phenanthrene-degrading bacteria in Puget Sound sediments were enumerated by most-probable-number enumeration procedures. Sediments from a creosote-contaminated Environmental Protection Agency Superfund Site (Eagle Harbor) contained from 10(4) to 10(7) polycyclic aromatic hydrocarbon (PAH)-degrading bacteria g (dry weight) of sediment-1, whereas the concentration at an uncontaminated site ranged from 10(3) to 10(4) g of sediment(-1). Isolates of PAH-degrading bacteria were obtained from these most-probable-number tubes as well as from sediment samples from noncontaminated sites and from bioreactors enriched with PAHs. The 18 resulting strains were grouped by whole-cell fatty acid analysis into two subgroups. The larger group of strains belonged to the newly described genus Cycloclasticus, whereas the other group contained members of the genus Vibrio. The Cycloclasticus group seems to be widespread in noncontaminated sediments. PAH degradation was confirmed in selected strains on the basis of removal of phenanthrene from growing cultures.
通过最大可能数计数法对普吉特海湾沉积物中降解萘和菲的细菌进行了计数。来自一个受杂酚油污染的美国环境保护局超级基金场地（鹰港）的沉积物每克（干重）含有10⁴至10⁷个降解多环芳烃（PAH）的细菌，而在一个未受污染场地的浓度范围为每克沉积物10³至10⁴个。从这些最大可能数试管以及未受污染场地的沉积物样本和富含多环芳烃的生物反应器中获得了多环芳烃降解细菌的分离株。通过全细胞脂肪酸分析将得到的18株菌株分为两个亚组。较大的一组菌株属于新描述的环裂菌属，而另一组包含弧菌属的成员。环裂菌属组似乎在未受污染的沉积物中广泛存在。根据生长培养物中菲的去除情况，在选定的菌株中证实了多环芳烃的降解。
