van Netten J P, Thornton I G, Carlyle S J, Brigden M L, Coy P, Goodchild N L, Gallagher S, George E J
Department of Pathology, Royal Jubilee Hospital, Victoria, British Columbia, Canada.
Eur J Cancer Clin Oncol. 1987 Sep;23(9):1337-42. doi: 10.1016/0277-5379(87)90117-9.
A multiple microsample analysis of estrogen receptors (ER) was performed on 81 samples obtained from 21 human breast cancers using a biochemical (BC) and immunohistochemical (IHC) method. Qualitative agreement occurred in 96% of the cases. Using both the percentage of cells staining specifically for ER as well as the intensity of staining (Histoscore) a semiquantitative correlation between the two analyses was obtained. Large variations in cellularity and the percentage of cells that stained specifically for ER existed within individual tumors. In most cases the variation in intensity of staining was minimal. Both the level and distribution of ER in different areas of individual tumors were calculated using a correction for differences in cellularity amongst samples. Heterogeneity in ER level as well as distribution was found in some tumors. In addition, a "checkerboard" type of staining with intermixed ER positive and ER negative cells was observed. This combined BC/IHC method may provide specific information about intratumor ER heterogeneity not available from either method alone.
使用生化（BC）和免疫组织化学（IHC）方法，对从21例人类乳腺癌中获取的81个样本进行了雌激素受体（ER）的多重微量样本分析。96%的病例出现了定性一致。利用特异性ER染色细胞的百分比以及染色强度（组织评分），获得了两种分析之间的半定量相关性。单个肿瘤内细胞数量和特异性ER染色细胞的百分比存在很大差异。在大多数情况下，染色强度的变化最小。使用样本间细胞数量差异校正，计算了单个肿瘤不同区域的ER水平和分布。在一些肿瘤中发现了ER水平和分布的异质性。此外，还观察到一种ER阳性和ER阴性细胞混合的“棋盘”式染色。这种BC/IHC联合方法可能提供关于肿瘤内ER异质性的特定信息，而单独使用任何一种方法都无法获得这些信息。
