Schalken J A, Roebroek A J, Oomen P P, Wagenaar S S, Debruyne F M, Bloemers H P, Van de Ven W J
Department of Urology, University of Nijmegen, The Netherlands.
J Clin Invest. 1987 Dec;80(6):1545-9. doi: 10.1172/JCI113240.
The recently discovered fur gene encodes a membrane-associated protein with a recognition function. To further characterize the gene, we studied its expression by Northern blot analysis using poly(A)-selected RNA from a variety of organs of African green monkey and rat. The fur gene appeared to be differentially expressed, relatively high levels of fur mRNA being present in specimens of liver and kidney, low levels in brain, spleen, and thymus, and very low levels in heart muscle, lung, and testis. mRNA levels in specimens of human lung tissue without neoplastic lesions were also very low. Similar analyses of primary human lung carcinomas of different histopathological types revealed a highly selective and strong elevation of fur expression in nonsmall cell lung carcinomas, but not in small cell lung carcinomas. These results indicate that fur expression can be used to discriminate between these two types of human lung cancer.
最近发现的fur基因编码一种具有识别功能的膜相关蛋白。为了进一步表征该基因，我们使用来自非洲绿猴和大鼠各种器官的聚腺苷酸（poly(A)）选择的RNA，通过Northern印迹分析研究了其表达情况。fur基因似乎存在差异表达，肝脏和肾脏标本中存在相对高水平的fur mRNA，大脑、脾脏和胸腺中水平较低，心肌、肺和睾丸中水平极低。无肿瘤病变的人肺组织标本中的mRNA水平也非常低。对不同组织病理学类型的原发性人肺癌进行的类似分析显示，非小细胞肺癌中fur表达高度选择性且强烈升高，而小细胞肺癌中则没有。这些结果表明，fur表达可用于区分这两种类型的人类肺癌。
