Campadelli-Fiume G, Arsenakis M, Farabegoli F, Roizman B
Section of Microbiology and Virology, University of Bologna, Italy.
J Virol. 1988 Jan;62(1):159-67. doi: 10.1128/JVI.62.1.159-167.1988.
The BJ cell line which constitutively expresses herpes simplex virus 1 glycoprotein D is resistant to infection with herpes simplex viruses. Analysis of clonal lines indicated that resistance to superinfecting virus correlates with the expression of glycoprotein D. Resistance was not due to a failure of attachment to cells, since the superinfecting virus absorbed to the BJ cells. Electron microscopic studies showed that the virions are juxtaposed to coated pits and are then taken up into endocytic vesicles. The virus particles contained in the vesicles were in various stages of degradation. Viral DNA that reached the nucleus was present in fewer copies per BJ cell than that in the parental BHKtk- cells infected at the same multiplicity. Moreover, unlike the viral DNA in BHKtk- cells which was amplified, that in BJ cells decreased in copy number. The results suggest that the glycoprotein D expressed in the BJ cell line interfered with fusion of the virion envelope with the plasma membrane but not with the adsorption of the virus to cells and that the viral proteins that mediate adsorption to and fusion of membranes appear to be distinct.
持续表达单纯疱疹病毒1型糖蛋白D的BJ细胞系对单纯疱疹病毒感染具有抗性。对克隆细胞系的分析表明，对再次感染病毒的抗性与糖蛋白D的表达相关。抗性并非由于无法附着于细胞，因为再次感染的病毒可吸附至BJ细胞。电子显微镜研究显示，病毒粒子与有被小窝并列，随后被摄入内吞小泡。小泡中所含的病毒颗粒处于不同的降解阶段。到达细胞核的病毒DNA，每个BJ细胞中的拷贝数比以相同感染复数感染的亲代BHKtk-细胞中的少。此外，与BHKtk-细胞中被扩增的病毒DNA不同，BJ细胞中的病毒DNA拷贝数减少。结果表明，BJ细胞系中表达的糖蛋白D干扰了病毒粒子包膜与质膜的融合，但不影响病毒对细胞的吸附，并且介导病毒吸附和膜融合的病毒蛋白似乎是不同的。
