Key Laboratory on Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.
Methods Appl Fluoresc. 2017 Mar 1;5(1):015003. doi: 10.1088/2050-6120/aa5e2b.
The detection of tetracycline is of great significance because of its damaging effects on human health, such as renal toxicity and hemolytic anemia. Any release of tetracycline into the surrounding environment can produce bacterial drug resistance. We develop a new sensitive and selective detection approach for tetracycline in complex water samples by preparing europium-doped carbon quantum dots (Eu-CQDs) through a simple and rapid carbonization method operating at 200 °C for 5 min. The Eu-CQDs are characterized by blue photoluminescence, excitation-wavelength-dependent emission and excellent stability. Importantly, the fluorescence of the Eu-CQDs can be quenched efficiently by tetracycline, based on the strong inner filter effect mechanism between Eu-CQDs and tetracycline, making the fluorescence intensity ratio (I /I) of the Eu-CQDs at 465 nm correlate linearly with the concentration of tetracycline in the range of 0.5-200 μM, with a limit of detection of 0.3 μM. This shows the broad applicability of the Eu-CQDs in pursuing the concepts of simplicity and specificity for analytical purposes.
四环素的检测具有重要意义，因为它会对人类健康造成损害，如肾毒性和溶血性贫血。四环素在周围环境中的任何释放都会产生细菌耐药性。我们通过在 200°C 下快速碳化 5 分钟来制备铕掺杂碳量子点（Eu-CQDs），从而开发出一种用于复杂水样中四环素的新型灵敏和选择性检测方法。Eu-CQDs 具有蓝色光致发光、激发波长依赖性发射和优异的稳定性。重要的是，基于 Eu-CQDs 和四环素之间的强内滤效应机制，四环素可以有效地猝灭 Eu-CQDs 的荧光，使 Eu-CQDs 在 465nm 处的荧光强度比（I / I）与四环素的浓度在 0.5-200 μM 的范围内呈线性相关，检测限为 0.3 μM。这表明 Eu-CQDs 在追求简单性和特异性分析概念方面具有广泛的适用性。
