Pandey Saurabh, Walpole Carina, Cabot Peter J, Shaw Paul N, Batra Jyotsna, Hewavitharana Amitha K
School of Pharmacy, The University of Queensland, Brisbane, Queensland, Australia; School of Biomedical Sciences, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia; Australian Prostate Cancer Research Centre-Qld, Translational Research Institute, Queensland University of Technology, Brisbane, Queensland, Australia.
School of Biomedical Sciences, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia; Australian Prostate Cancer Research Centre-Qld, Translational Research Institute, Queensland University of Technology, Brisbane, Queensland, Australia.
Biomed Pharmacother. 2017 May;89:515-523. doi: 10.1016/j.biopha.2017.02.050. Epub 2017 Feb 27.
Prostate cancer (PCa) is the leading cause of cancer related deaths in men. Carica papaya is a popular tropical plant that has been traditionally used for its nutritional and medicinal properties.
We investigated the anti-proliferative responses of papaya leaf juice (LJP) and its various extracts ("biological"- in vitro digested, "physical"- size exclusion, and "chemical"-solvent extraction) on a range of cell lines representing benign hyperplasia, tumorigenic and normal cells of prostate origin.
Time course analysis (by 24h, 48h and 72h) of LJP (1-0.1mg/mL) before and after in vitro digestion, and of molecular weight based fractions of LJP showed anti-proliferative responses. The medium polarity fraction of LJP (0.03-0.003mg/mL) after 72h exposure showed potent growth inhibitory (IC=0.02-0.07mg/mL) and cytotoxic activities on all prostate cells, with the exception of the normal (RWPE-1 and WPMY-1) cells. Flow cytometry analysis showed S phase cell cycle arrest and apoptosis as a possible mechanism for these activities. Medium polar fraction of LJP also inhibited migration and adhesion of metastatic PC-3 cells.
This is the first report suggesting selective anti-proliferative and anti-metastatic attributes of LJP extract against prostatic diseases, including PCa.
前列腺癌（PCa）是男性癌症相关死亡的主要原因。番木瓜是一种广受欢迎的热带植物，传统上因其营养和药用特性而被使用。
我们研究了木瓜叶汁（LJP）及其各种提取物（“生物”——体外消化、“物理”——尺寸排阻和“化学”——溶剂萃取）对一系列代表前列腺来源的良性增生、致瘤和正常细胞的细胞系的抗增殖反应。
对体外消化前后的LJP（1 - 0.1mg/mL）以及基于分子量的LJP组分进行时间进程分析（24小时、48小时和72小时），显示出抗增殖反应。LJP的中等极性组分（0.03 - 0.003mg/mL）在暴露72小时后，对所有前列腺细胞（正常细胞（RWPE - 1和WPMY - 1）除外）显示出强大的生长抑制（IC = 0.02 - 0.07mg/mL）和细胞毒性活性。流式细胞术分析表明S期细胞周期停滞和凋亡可能是这些活性的机制。LJP的中等极性组分还抑制转移性PC - 3细胞的迁移和黏附。
这是首次报道表明LJP提取物对包括PCa在内的前列腺疾病具有选择性抗增殖和抗转移特性。
