Seligmann B, Patel K, Haston W O, Rediske J J
Research Department, CIBA-GEIGY Corporation, Summit, NJ 07901.
Agents Actions. 1987 Aug;21(3-4):375-8. doi: 10.1007/BF01966520.
Simultaneous measurements of the calcium rise, membrane potential change, and 90 degrees light scatter (shape change) responses exhibited by neutrophils upon activation, can be obtained with identical result as that obtained when independently performing each measurement. The putative intracellular mediator diacylglycerol depolarizes membrane potential and causes a decrease in light scatter. Leukotriene B4 causes a rise in calcium and a decrease in light scatter. The chemotactic peptide, N-formylmethionyl-leucyl-phenylalanine, causes a depolarization of membrane potential, a calcium rise, and a decrease in light scatter. The fura 2 measurements of intracellular free calcium indicate that the calcium concentration of unstimulated cells is much lower than previously thought based on quin 2 studies.
同时测量中性粒细胞激活时出现的钙升高、膜电位变化和90度光散射（形态变化）反应，可获得与独立进行每项测量时相同的结果。假定的细胞内介质二酰基甘油使膜电位去极化并导致光散射减少。白三烯B4导致钙升高和光散射减少。趋化肽N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸导致膜电位去极化、钙升高和光散射减少。用fura 2测量细胞内游离钙表明，未受刺激细胞的钙浓度比基于quin 2研究先前认为的要低得多。
