DeLap R J, Rush M G
Proc Natl Acad Sci U S A. 1978 Dec;75(12):5855-9. doi: 10.1073/pnas.75.12.5855.
Small circular DNAs ranging in contour length from 0.06 to 3.5 micrometers have been isolated from bursas of 19-day chicken embryos and 4- to 5-week-old chickens. Small circular DNA is present in bursas of 19-day embryos at approximately 0.2 molecules per cell and is very heterogeneous, lacking distinct size classes; most molecules have contour lengths of less than 0.04 micrometers. In contrast, small circular DNA is present in bursas of 4- to 5-week old chickens at about 4 molecules per cell, and although this DNA is still heterogeneous, it contains a major distinct class of molecules 0.8 micrometers in size. These small circular DNAs may be products of developmental gene rearrangements occurring in the chromosomal DNA of lymphocytes in the bursa.
已从19日龄鸡胚和4至5周龄鸡的法氏囊中分离出轮廓长度在0.06至3.5微米之间的小环状DNA。19日龄胚胎的法氏囊中存在小环状DNA，每个细胞约有0.2个分子，且非常不均一，缺乏明显的大小类别；大多数分子的轮廓长度小于0.04微米。相比之下，4至5周龄鸡的法氏囊中存在小环状DNA，每个细胞约有4个分子，虽然这种DNA仍然不均一，但它包含一类主要的大小为0.8微米的独特分子。这些小环状DNA可能是法氏囊中淋巴细胞染色体DNA发生发育基因重排的产物。
