多种机制在中国仓鼠卵巢细胞中产生染色体外环状DNA。
Multiple mechanisms generate extrachromosomal circular DNA in Chinese hamster ovary cells.
作者信息
Stanfield S W, Helinski D R
出版信息
Nucleic Acids Res. 1986 Apr 25;14(8):3527-38. doi: 10.1093/nar/14.8.3527.
Abstract
Seven cloned small circular DNA molecules from CHO cells were sequenced and examined for the presence of homologies to each other and to a number of other functional sequences present in transposable elements, retroviruses, mammalian repeat sequences, and introns. The sequences of the CHO cell circular DNA molecules did not reveal common structural features that could explain their presence in the circular DNA population. A gene bank was constructed for CHO chromosomal DNA and sequences homologous to two of the seven small circular DNA molecules were isolated and sequenced. The nucleotide sequences present at the junction of circular and chromosomal DNA suggest that a recombination process involving homologous pairing may have been involved in the generation of one, but not the other, of the two circular DNA molecules.
摘要
对来自中国仓鼠卵巢（CHO）细胞的七个克隆的小环状DNA分子进行了测序，并检查了它们彼此之间以及与转座元件、逆转录病毒、哺乳动物重复序列和内含子中存在的许多其他功能序列的同源性。CHO细胞环状DNA分子的序列未揭示出可解释它们在环状DNA群体中存在的共同结构特征。构建了CHO染色体DNA的基因文库，并分离和测序了与七个小环状DNA分子中的两个同源的序列。环状和染色体DNA交界处的核苷酸序列表明，涉及同源配对的重组过程可能参与了两个环状DNA分子中一个而非另一个的产生。
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