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干扰素作用：双链RNA抑制蛋白质合成的两条不同途径。

Interferon action: two distinct pathways for inhibition of protein synthesis by double-stranded RNA.

作者信息

Farrell P J, Sen G C, Dubois M F, Ratner L, Slattery E, Lengyel P

出版信息

Proc Natl Acad Sci U S A. 1978 Dec;75(12):5893-7. doi: 10.1073/pnas.75.12.5893.

DOI:10.1073/pnas.75.12.5893
PMID:282611
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC393082/
Abstract

Double-stranded RNA inhibits protein synthesis in at least two ways. It activates a protein kinase that blocks peptide chain initiation by phosphorylating the peptide chain initiation factor eIF-2 and also activates an endonuclease that inactivates different mRNAs at different rates. The protein kinase and the endonuclease have been partially purified from interferon-treated Ehrlich ascites tumor cells. The 2',5'-oligoadenylates [pppA(2'p5'A)n], found found earlier to be mediators in the activation of the endonuclease by double-stranded RNA, are not mediators in the activation of the protein kinase by double-stranded RNA.

摘要

双链RNA至少通过两种方式抑制蛋白质合成。它激活一种蛋白激酶，该激酶通过磷酸化肽链起始因子eIF-2来阻断肽链起始，并且还激活一种核酸内切酶，该核酸内切酶以不同速率使不同的mRNA失活。蛋白激酶和核酸内切酶已从经干扰素处理的艾氏腹水瘤细胞中部分纯化出来。2',5'-寡腺苷酸[pppA(2'p5'A)n]，此前发现它是双链RNA激活核酸内切酶的介质，但不是双链RNA激活蛋白激酶的介质。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0060/393082/cd41e0e660b8/pnas00022-0159-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0060/393082/869ae65f2aea/pnas00022-0158-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0060/393082/cd41e0e660b8/pnas00022-0159-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0060/393082/869ae65f2aea/pnas00022-0158-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0060/393082/cd41e0e660b8/pnas00022-0159-a.jpg

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Interferon action: two distinct pathways for inhibition of protein synthesis by double-stranded RNA.干扰素作用：双链RNA抑制蛋白质合成的两条不同途径。
Proc Natl Acad Sci U S A. 1978 Dec;75(12):5893-7. doi: 10.1073/pnas.75.12.5893.
2
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Interferon, double-stranded RNA, and RNA degradation. Isolation of homogeneous pppA(2'p5'A)n-1 synthetase from Ehrlich ascites tumor cells.干扰素、双链RNA与RNA降解。从艾氏腹水瘤细胞中分离出均一的pppA(2'p5'A)n-1合成酶。
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Differential effects of two interferon-induced translational inhibitors on initiation of protein synthesis.两种干扰素诱导的翻译抑制剂对蛋白质合成起始的不同作用。
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Double-stranded RNA-dependent phosphorylation of protein P1 and eukaryotic initiation factor 2 alpha does not correlate with protein synthesis inhibition in a cell-free system from interferon-treated mouse L cells.在经干扰素处理的小鼠L细胞的无细胞体系中，蛋白质P1和真核起始因子2α的双链RNA依赖性磷酸化与蛋白质合成抑制不相关。
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J Mol Biol. 1973 May 25;76(3):379-403. doi: 10.1016/0022-2836(73)90511-1.
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Increased sensitivity of cell-free protein synthesis to double-stranded RNA after interferon treatment.干扰素处理后无细胞蛋白质合成对双链RNA的敏感性增加。
Nature. 1974 Jul 5;250(461):57-9. doi: 10.1038/250057a0.
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Increased endonuclease activity in an extract from mouse Ehrlich ascites tumor cells which had been treated with a partially purified interferon preparation: dependence of double-stranded RNA;
PLoS Pathog. 2021 Dec 16;17(12):e1010091. doi: 10.1371/journal.ppat.1010091. eCollection 2021 Dec.
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Loss of the Nuclear Protein RTF2 Enhances Influenza Virus Replication.核蛋白 RTF2 的缺失增强了流感病毒的复制。
J Virol. 2020 Oct 27;94(22). doi: 10.1128/JVI.00319-20.
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Early type I IFN blockade improves the efficacy of viral vaccines.早期 I 型干扰素阻断可提高病毒疫苗的疗效。
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Prevention of dsRNA-induced interferon signaling by AGO1x is linked to breast cancer cell proliferation.AGO1x 通过抑制 dsRNA 诱导的干扰素信号转导促进乳腺癌细胞增殖。
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Nature. 1976 Nov 25;264(5584):370-3. doi: 10.1038/264370a0.
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