Buthod L M, Schindler N L, Rogers H W
Indiana University School of Medicine, Evansville Center for Medical Education, Indiana.
J Virol Methods. 1987 Oct;18(1):37-46. doi: 10.1016/0166-0934(87)90108-x.
Herpes simplex virus (HSV) is one of the most commonly identified viruses in the clinical laboratory. HSV is of clinical consequence because of its ability to produce such life-threatening infections as encephalitis and neonatal disease. A variety of cell lines are currently being used for detection of HSV in cell culture. This study compared several cell lines simultaneously in a 96-well cell culture system to determine which lines demonstrated viral CPE most quickly following infection. In considering speed at which viral CPE is demonstrated, our results showed that primary rabbit kidney cells surpassed all other cell lines at 48 h post-viral infection. Alternative cell lines demonstrated to be suitable by this study were human fetal foreskin, human embryonic lung, and Vero cells.
单纯疱疹病毒（HSV）是临床实验室中最常检测到的病毒之一。HSV具有临床意义，因为它能够引发如脑炎和新生儿疾病等危及生命的感染。目前，多种细胞系被用于细胞培养中HSV的检测。本研究在96孔细胞培养系统中同时比较了几种细胞系，以确定哪些细胞系在感染后能最快出现病毒致细胞病变效应（CPE）。在考虑出现病毒CPE的速度时，我们的结果表明，原代兔肾细胞在病毒感染后48小时超过了所有其他细胞系。本研究证明合适的替代细胞系为人胎儿包皮细胞、人胚肺细胞和非洲绿猴肾细胞（Vero细胞）。
