使用Cas9-抗生物素蛋白/生物素供体DNA系统高效生成携带纯合双loxP等位基因的小鼠。 - Suppr

Efficient generation of mice carrying homozygous double-floxp alleles using the Cas9-Avidin/Biotin-donor DNA system.

作者信息

Ma Ming, Zhuang Fengfeng, Hu Xiongbing, Wang Bolun, Wen Xian-Zi, Ji Jia-Fu, Xi Jianzhong Jeff

机构信息

Department of Biomedical Engineering, State Key Laboratory of Natural and Biomimetic Drugs, College of Engineering, Peking University, Beijing, China.

Beijing Viewsolid Biotech Co. Ltd, Beijing 100071, China.

出版信息

Cell Res. 2017 Apr;27(4):578-581. doi: 10.1038/cr.2017.29. Epub 2017 Mar 7.

DOI:10.1038/cr.2017.29

PMID:28266543

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5385615/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc74/5385615/e8a3d0e1cdff/cr201729f1.jpg

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Efficient generation of mice carrying homozygous double-floxp alleles using the Cas9-Avidin/Biotin-donor DNA system.

作者信息

Ma Ming, Zhuang Fengfeng, Hu Xiongbing, Wang Bolun, Wen Xian-Zi, Ji Jia-Fu, Xi Jianzhong Jeff

机构信息

Department of Biomedical Engineering, State Key Laboratory of Natural and Biomimetic Drugs, College of Engineering, Peking University, Beijing, China.

Beijing Viewsolid Biotech Co. Ltd, Beijing 100071, China.

出版信息

Cell Res. 2017 Apr;27(4):578-581. doi: 10.1038/cr.2017.29. Epub 2017 Mar 7.

DOI:10.1038/cr.2017.29

PMID:28266543

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5385615/

Abstract

摘要

相似文献

Efficient generation of mice carrying homozygous double-floxp alleles using the Cas9-Avidin/Biotin-donor DNA system.

Cell Res. 2017 Apr;27(4):578-581. doi: 10.1038/cr.2017.29. Epub 2017 Mar 7.

Super-Mendelian inheritance mediated by CRISPR-Cas9 in the female mouse germline.

Nature. 2019 Feb;566(7742):105-109. doi: 10.1038/s41586-019-0875-2. Epub 2019 Jan 23.

Conditional expression of Lodavin, an avidin-tagged LDL receptor, for biotin-mediated applications in vivo.

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Biochemistry. 1977 Apr 5;16(7):1364-70. doi: 10.1021/bi00626a020.

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FEBS Lett. 1993 May 3;322(1):47-50. doi: 10.1016/0014-5793(93)81108-c.

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Biotechnol Appl Biochem. 1991 Oct;14(2):192-201.

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Nucleic Acids Res. 2021 Apr 6;49(6):3546-3556. doi: 10.1093/nar/gkab123.

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Proc Natl Acad Sci U S A. 1979 Feb;76(2):676-80. doi: 10.1073/pnas.76.2.676.

Bradavidin II from Bradyrhizobium japonicum: a new avidin-like biotin-binding protein.

Biochim Biophys Acta. 2008 Jul-Aug;1784(7-8):1002-10. doi: 10.1016/j.bbapap.2008.04.010. Epub 2008 Apr 30.

Recognition of oxidatively modified bases within the biotin-binding site of avidin.

J Mol Biol. 2006 Mar 17;357(1):263-74. doi: 10.1016/j.jmb.2005.12.054. Epub 2006 Jan 6.

引用本文的文献

Enhancing Specificity, Precision, Accessibility, Flexibility, and Safety to Overcome Traditional CRISPR/Cas Editing Challenges and Shape Future Innovations.

Adv Sci (Weinh). 2025 Jul;12(28):e2416331. doi: 10.1002/advs.202416331. Epub 2025 Jun 23.

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Nat Commun. 2025 May 16;16(1):4569. doi: 10.1038/s41467-025-59790-3.

Fine-Tuning Homology-Directed Repair (HDR) for Precision Genome Editing: Current Strategies and Future Directions.

Int J Mol Sci. 2025 Apr 25;26(9):4067. doi: 10.3390/ijms26094067.

Autophagy induction enhances homologous recombination-associated CRISPR-Cas9 gene editing.

Nucleic Acids Res. 2025 Apr 10;53(7). doi: 10.1093/nar/gkaf258.

Cas9-Rep fusion tethers donor DNA in vivo and boosts the efficiency of HDR-mediated genome editing.

Plant Biotechnol J. 2025 Jun;23(6):2006-2017. doi: 10.1111/pbi.70036. Epub 2025 Mar 5.

Engineering Base Changes and Epitope-Tagged Alleles in Mice Using Cas9 RNA-Guided Nuclease.

Curr Protoc. 2025 Feb;5(2):e70109. doi: 10.1002/cpz1.70109.

Enhancing homology-directed repair efficiency with HDR-boosting modular ssDNA donor.

Nat Commun. 2024 Aug 10;15(1):6843. doi: 10.1038/s41467-024-50788-x.

An Irak1-Mecp2 tandem duplication mouse model for the study of MECP2 duplication syndrome.

Dis Model Mech. 2024 Jul 1;17(7). doi: 10.1242/dmm.050528. Epub 2024 Jul 23.

Exonuclease editor promotes precision of gene editing in mammalian cells.

BMC Biol. 2024 May 20;22(1):119. doi: 10.1186/s12915-024-01918-w.

CRISPR Ribonucleoprotein-Mediated Precise Editing of Multiple Genes in Porcine Fibroblasts.

Animals (Basel). 2024 Feb 18;14(4):650. doi: 10.3390/ani14040650.

本文引用的文献

RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency.

Nat Commun. 2016 Jan 28;7:10548. doi: 10.1038/ncomms10548.

Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA.

Nat Biotechnol. 2016 Mar;34(3):339-44. doi: 10.1038/nbt.3481. Epub 2016 Jan 20.

ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes.

Nat Commun. 2016 Jan 20;7:10431. doi: 10.1038/ncomms10431.

A possible aid in targeted insertion of large DNA elements by CRISPR/Cas in mouse zygotes.

Genesis. 2016 Feb;54(2):65-77. doi: 10.1002/dvg.22914. Epub 2016 Jan 17.

Highly efficient CRISPR/HDR-mediated knock-in for mouse embryonic stem cells and zygotes.

Biotechniques. 2015 Oct 1;59(4):201-2, 204, 206-8. doi: 10.2144/000114339. eCollection 2015 Oct.

Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining.

Nat Biotechnol. 2015 May;33(5):538-42. doi: 10.1038/nbt.3190. Epub 2015 Mar 23.

Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery.

Elife. 2014 Dec 15;3:e04766. doi: 10.7554/eLife.04766.

Development and applications of CRISPR-Cas9 for genome engineering.

Cell. 2014 Jun 5;157(6):1262-1278. doi: 10.1016/j.cell.2014.05.010.

CRISPR-Cas systems for editing, regulating and targeting genomes.

Nat Biotechnol. 2014 Apr;32(4):347-55. doi: 10.1038/nbt.2842. Epub 2014 Mar 2.

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Cell Stem Cell. 2013 Dec 5;13(6):659-62. doi: 10.1016/j.stem.2013.10.016.

Efficient generation of mice carrying homozygous double-floxp alleles using the Cas9-Avidin/Biotin-donor DNA system.

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Efficient generation of mice carrying homozygous double-floxp alleles using the Cas9-Avidin/Biotin-donor DNA system.

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