用于软组织工程应用的负载埃洛石纳米管的结冷胶水凝胶的研究进展。
Insight into halloysite nanotubes-loaded gellan gum hydrogels for soft tissue engineering applications.
机构信息
Dept. of Chemistry, University of Bari Aldo Moro, Via E. Orabona 4, Bari, 70126, Italy.
School of Mechanical and Systems Engineering, Newcastle University, Stephenson Building, Claremont Road, Newcastle upon Tyne, NE1 7RU, UK.
出版信息
Carbohydr Polym. 2017 May 1;163:280-291. doi: 10.1016/j.carbpol.2017.01.064. Epub 2017 Jan 20.
A tri-component hydrogel, based on gellan gum (GG), glycerol (Gly) and halloysite nanotubes (HNT), is proposed in this work for soft tissue engineering applications. The FDA-approved GG polysaccharide has been recently exploited as biomaterial because its biomimetic features. Gly is added as molecular spacer to improve hydrogel viscosity and mechanical properties. HNT incorporation within the hydrogel offers the versatility to improve the GG-Gly biocompatibility with potential incorporation of target biomolecules. In this work, hydrogels with different composition ratios are physically crosslinked for tuning physico-mechanical properties. An accurate physico-chemical characterization is reported. HNT addition leads to a water uptake decrease of 30-35% and tuneable mechanical properties with a compressive Young's modulus ranging between 20 and 75kPa. Finally, in vitro study with human fibroblasts on GG-Gly hydrogels loaded with 25% HNT offered the higher metabolic activities and cell survival up to 7days of incubation.
本文提出了一种基于结冷胶（GG）、甘油（Gly）和海泡石纳米管（HNT）的三组分水凝胶，可用于软组织工程应用。最近，FDA 批准的 GG 多糖已被用作生物材料，因为其具有仿生特性。Gly 作为分子间隔物添加，以提高水凝胶的粘度和机械性能。HNT 的加入使水凝胶具有多功能性，可以提高 GG-Gly 的生物相容性，并且有可能加入目标生物分子。在这项工作中，不同组成比例的水凝胶通过物理交联进行物理交联，以调整物理化学性质。报告了准确的物理化学特性。HNT 的加入导致水吸收减少 30-35%，并可调节压缩杨氏模量在 20-75kPa 之间的机械性能。最后，在含有 25%HNT 的 GG-Gly 水凝胶上进行的人成纤维细胞体外研究表明，在孵育 7 天的情况下，细胞代谢活性和存活率更高。
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