细胞色素c对重组NADH：细胞色素c还原酶中泛醌池氧化还原状态的限速作用。
The effect of rate limitation by cytochrome c on the redox state of the ubiquinone pool in reconstituted NADH: cytochrome c reductase.
作者信息
Reed J S, Ragan C I
机构信息
Department of Biochemistry, University of Southampton, U.K.
出版信息
Biochem J. 1987 Nov 1;247(3):657-62. doi: 10.1042/bj2470657.
Abstract
The kinetic model of Ragan & Cottingham [(1985) Biochim. Biophys. Acta 811, 13-31] for electron transport through a mobile pool of quinone predicts that, under certain conditions, the normal linear dependence of electron flow on the degree of reduction (or oxidation) of the quinone should no longer be found. These conditions can be met by reconstituted NADH: cytochrome c reductase (Complex I-III from bovine heart) when electron flow is rate-limited by a low concentration of cytochrome c. We show that, in such a system, the dependence of activity (varied by inhibition with rotenone) on the steady-state level of quinone reduction is indeed non-linear and very closely accounted for by the theory.
摘要
拉根和科廷厄姆[（1985年）《生物化学与生物物理学报》811卷，第13 - 31页]提出的关于电子通过醌的流动池进行传输的动力学模型预测，在某些条件下，电子流对醌的还原（或氧化）程度的正常线性依赖关系将不再存在。当电子流受低浓度细胞色素c的速率限制时，重组的NADH：细胞色素c还原酶（来自牛心的复合体I - III）可以满足这些条件。我们表明，在这样一个系统中，活性（通过鱼藤酮抑制来改变）对醌还原稳态水平的依赖性确实是非线性的，并且该理论能非常精确地解释这一现象。
相似文献
1
The effect of rate limitation by cytochrome c on the redox state of the ubiquinone pool in reconstituted NADH: cytochrome c reductase.细胞色素c对重组NADH：细胞色素c还原酶中泛醌池氧化还原状态的限速作用。
Biochem J. 1987 Nov 1;247(3):657-62. doi: 10.1042/bj2470657.
2
3
Production of superoxide radicals and hydrogen peroxide by NADH-ubiquinone reductase and ubiquinol-cytochrome c reductase from beef-heart mitochondria.来自牛心线粒体的NADH-泛醌还原酶和泛醇-细胞色素c还原酶产生超氧自由基和过氧化氢。
Arch Biochem Biophys. 1977 Apr 30;180(2):248-57. doi: 10.1016/0003-9861(77)90035-2.
4
5
Enzymology of ubiquinone-utilizing electron transfer complexes in nonionic detergent.非离子去污剂中利用泛醌的电子传递复合物的酶学
Eur J Biochem. 1979 Aug 15;99(1):151-60. doi: 10.1111/j.1432-1033.1979.tb13241.x.
6
Cytochrome c-mediated electron transfer between ubiquinol-cytochrome c reductase and cytochrome c oxidase. Kinetic evidence for a mobile cytochrome c pool.细胞色素c介导的泛醇-细胞色素c还原酶与细胞色素c氧化酶之间的电子传递。细胞色素c可移动池的动力学证据。
Biochem J. 1984 Jan 15;217(2):551-60. doi: 10.1042/bj2170551.
7
Reversible inhibition of electron transfer in the ubiquinol. Cytochrome c reductase segment of the mitochondrial respiratory chain in hibernating ground squirrels.冬眠地松鼠线粒体呼吸链中泛醇-细胞色素c还原酶部分电子传递的可逆抑制作用。
FEBS Lett. 1990 Apr 9;263(1):73-6. doi: 10.1016/0014-5793(90)80708-q.
8
Generation of superoxide anion by the NADH dehydrogenase of bovine heart mitochondria.牛心线粒体NADH脱氢酶产生超氧阴离子。
Biochem J. 1980 Nov 1;191(2):421-7. doi: 10.1042/bj1910421.
9
The effects of lipid fluidity on the rotational diffusion of complex I and complex III in reconstituted NADH-cytochrome c oxidoreductase.脂质流动性对重组NADH-细胞色素c氧化还原酶中复合物I和复合物III旋转扩散的影响。
Biochim Biophys Acta. 1982 Dec 8;693(1):113-24. doi: 10.1016/0005-2736(82)90477-1.
10
The relationship between electron flux and the redox poise of the quinone pool in plant mitochondria. Interplay between quinol-oxidizing and quinone-reducing pathways.植物线粒体中电子通量与醌池氧化还原平衡之间的关系。醌醇氧化途径与醌还原途径之间的相互作用。
Eur J Biochem. 1994 Dec 15;226(3):1071-8. doi: 10.1111/j.1432-1033.1994.01071.x.
引用本文的文献
1
Identifying Site-Specific Superoxide and Hydrogen Peroxide Production Rates From the Mitochondrial Electron Transport System Using a Computational Strategy.利用计算策略鉴定线粒体电子传递系统中特定部位的超氧化物和过氧化氢产生速率。
Function (Oxf). 2021 Sep 20;2(6):zqab050. doi: 10.1093/function/zqab050. eCollection 2021.
2
Mitochondria and Viral Infection: Advances and Emerging Battlefronts.线粒体与病毒感染：研究进展与新兴前沿领域。
mBio. 2022 Feb 22;13(1):e0209621. doi: 10.1128/mbio.02096-21. Epub 2022 Jan 25.
3
Analysis of a Functional Dimer Model of Ubiquinol Cytochrome c Oxidoreductase.泛醇细胞色素c氧化还原酶功能二聚体模型分析
Biophys J. 2017 Oct 3;113(7):1599-1612. doi: 10.1016/j.bpj.2017.08.018.
4
Mitochondrial deficits and abnormal mitochondrial retrograde axonal transport play a role in the pathogenesis of mutant Hsp27-induced Charcot Marie Tooth Disease.线粒体缺陷和异常的线粒体逆行轴突运输在突变型热休克蛋白27（Hsp27）诱导的夏科-马里-图斯病（Charcot Marie Tooth Disease）发病机制中起作用。
Hum Mol Genet. 2017 Sep 1;26(17):3313-3326. doi: 10.1093/hmg/ddx216.
5
Analysis of the kinetics and bistability of ubiquinol:cytochrome c oxidoreductase.分析泛醇：细胞色素 c 氧化还原酶的动力学和双稳态。
Biophys J. 2013 Jul 16;105(2):343-55. doi: 10.1016/j.bpj.2013.05.033.
6
Apoptosis Induced by Manganese on Neuronal SK-N-MC Cell Line: Endoplasmic Reticulum (ER) Stress and Mitochondria Dysfunction.锰诱导神经母细胞瘤SK-N-MC细胞系凋亡：内质网应激与线粒体功能障碍
Environ Health Toxicol. 2011;26:e2011017. doi: 10.5620/eht.2011.26.e2011017. Epub 2011 Dec 20.
7
Regulation of Alternative Pathway Activity in Plant Mitochondria : Deviations from Q-Pool Behavior during Oxidation of NADH and Quinols.植物线粒体中替代途径活性的调节：在 NADH 和醌氧化过程中偏离 Q-池行为。
Plant Physiol. 1991 Mar;95(3):948-53. doi: 10.1104/pp.95.3.948.
8
Kinetic and regulatory aspects of the function of the alternative oxidase in plant respiration.植物呼吸中交替氧化酶功能的动力学和调节方面
J Bioenerg Biomembr. 1995 Aug;27(4):387-96. doi: 10.1007/BF02110001.
本文引用的文献
1
Isolation of Mitochondria from Leaf Tissue of Panicum miliaceum, a NAD-Malic Enzyme Type C(4) Plant.从属于 NAD-Malic 酶类型 C(4)植物的小米叶片组织中分离线粒体。
Plant Physiol. 1983 Jan;71(1):24-9. doi: 10.1104/pp.71.1.24.
2
On the role of ubiquinone in the respiratory chain.论泛醌在呼吸链中的作用。
Biochim Biophys Acta. 1982 Apr 19;680(1):69-79. doi: 10.1016/0005-2728(82)90317-6.
3
Studies on reduced and oxidized coenzyme Q (ubiquinones). II. The determination of oxidation-reduction levels of coenzyme Q in mitochondria, microsomes and plasma by high-performance liquid chromatography.还原型和氧化型辅酶Q（泛醌）的研究。II. 用高效液相色谱法测定线粒体、微粒体和血浆中辅酶Q的氧化还原水平。
Biochim Biophys Acta. 1982 Feb 17;679(2):308-14. doi: 10.1016/0005-2728(82)90301-2.
4
Electron flux through the mitochondrial ubiquinone.通过线粒体泛醌的电子通量。
Biochim Biophys Acta. 1980 Dec 22;594(1):53-84. doi: 10.1016/0304-4173(80)90013-0.
5
Purification of three iron-sulfur proteins from the iron-protein fragment of mitochondrial NADH-ubiquinone oxidoreductase.从线粒体NADH-泛醌氧化还原酶的铁蛋白片段中纯化三种铁硫蛋白。
Biochemistry. 1982 May 11;21(10):2518-24. doi: 10.1021/bi00539a035.
6
Electron and proton transfers through quinones and cytochrome bc complexes.电子和质子通过醌类和细胞色素bc复合物的转移。
Biochim Biophys Acta. 1984 Apr 9;768(1):53-79. doi: 10.1016/0304-4173(84)90007-7.
7
The effects of lipid fluidity on the rotational diffusion of complex I and complex III in reconstituted NADH-cytochrome c oxidoreductase.脂质流动性对重组NADH-细胞色素c氧化还原酶中复合物I和复合物III旋转扩散的影响。
Biochim Biophys Acta. 1982 Dec 8;693(1):113-24. doi: 10.1016/0005-2736(82)90477-1.
8
A spin label study of the lipid boundary layer of mitochondrial NADH-ubiquinone oxidoreductase.线粒体NADH-泛醌氧化还原酶脂质边界层的自旋标记研究。
Biochim Biophys Acta. 1982 Dec 8;693(1):105-12. doi: 10.1016/0005-2736(82)90476-x.
9
Further evidence for the pool function of ubiquinone as derived from the inhibition of the electron transport by antimycin.抗霉素对电子传递的抑制作用所衍生的关于泛醌池功能的进一步证据。
Eur J Biochem. 1973 Nov 15;39(2):313-23. doi: 10.1111/j.1432-1033.1973.tb03129.x.
10
The kinetics of the redox reactions of ubiquinone related to the electron-transport activity in the respiratory chain.与呼吸链中电子传递活性相关的泛醌氧化还原反应动力学。
Eur J Biochem. 1973 Apr;34(2):358-68. doi: 10.1111/j.1432-1033.1973.tb02767.x.
Zotero 插件