Elbay Ahmet, Ozer Omer Faruk, Altinisik Muhammed, Elbay Arif Emre, Sezer Taha, Bayraktar Havvanur, Ozdemir Hakan
a Department of Ophthalmology , Bezmialem University School of Medicine , Istanbul , Turkey.
b Department of Biochemistry , Bezmialem University School of Medicine , Istanbul , Turkey.
Scand J Clin Lab Invest. 2017 May;77(3):223-227. doi: 10.1080/00365513.2017.1292539. Epub 2017 Feb 28.
We investigated serum and aqueous humor thiol/disulfide (T-D) homeostasis in patients with cataracts versus healthy controls. In total, 56 patients with cataracts and 49 healthy controls were enrolled in this case-control study. Serum total thiol (TT), native thiol (NT), and disulfide (DS) concentrations were determined using a novel automated measurement method. Additionally, DS/TT, DS/NT and NT/TT percentage ratios were compared between the groups. In comparison with the control group, serum NT levels and aqueous humor TT and NT levels were significantly lower (p < .05, p < .05 and p < .001, respectively), whereas serum and aqueous humor DS levels were significantly higher in cataract patients (p < .01 and p < .001). DS/TT and DS/NT ratios were significantly higher and the NT/TT ratio was lower in cataract patients in serum (p < .005) and aqueous humor samples (p < .001). In conclusion, serum T-D homeostasis may be useful as biochemical markers, indicating the role of oxidative stress in the development of cataracts. Further studies are needed to confirm the pathophysiological role of T-D homeostasis in cataractogenesis.
我们研究了白内障患者与健康对照者血清和房水中硫醇/二硫化物（T-D）的稳态。本病例对照研究共纳入了56例白内障患者和49名健康对照者。采用一种新型自动测量方法测定血清总硫醇（TT）、天然硫醇（NT）和二硫化物（DS）浓度。此外，还比较了两组之间DS/TT、DS/NT和NT/TT的百分比比值。与对照组相比，白内障患者血清NT水平以及房水TT和NT水平显著降低（分别为p < 0.05、p < 0.05和p < 0.001），而白内障患者血清和房水DS水平显著升高（p < 0.01和p < 0.001）。白内障患者血清（p < 0.005）和房水样本（p < 0.001）中DS/TT和DS/NT比值显著升高，NT/TT比值降低。总之，血清T-D稳态可能作为生化标志物，提示氧化应激在白内障发生发展中的作用。需要进一步研究来证实T-D稳态在白内障形成中的病理生理作用。
