Sagdik Haci Murat, Ucar Fatma, Tetikoglu Mehmet, Aktas Serdar, Ozcura Fatih, Kocak Havva, Neselioglu Salim, Eren Funda
Department of Ophthalmology, Dumlupinar University School of Medicine, Kutahya, Turkey.
Department of Clinical Biochemistry, Diskapi Yildirim Beyazit Training and Research Hospital, Ankara, Turkey.
Int Ophthalmol. 2018 Apr;38(2):655-661. doi: 10.1007/s10792-017-0513-7. Epub 2017 Apr 10.
The aim of this study was to determine plasma thiol-disulphide homoeostasis in patients with age-related cataract (ARC) and compare the results of the patients with healthy subjects. Plasma malondialdehyde (MDA) levels and catalase (CAT) activity were also investigated.
The study included 53 cataract patients and 52 healthy volunteers. Native thiol-disulphide exchanges were determined using a novel and automated method. CAT activity was determined using the method described by Aebi, and MDA levels were calculated using the thiobarbituric acid method.
Native thiol and total thiol levels were significantly lower in the cataract patients compared with the controls (p < 0.001, p = 0.002, respectively). The disulphide levels of the cataract patients were significantly higher than the controls (p = 0.002). The ratios of disulphide/native thiol and disulphide/total thiol were statistically higher in the cataract patients compared with the control group (p < 0.001, p < 0.001, respectively). Furthermore, CAT activity was significantly lower in the cataract patient group compared with the control group (p < 0.001), and MDA levels were insignificantly higher in the patient group (p = 0.581).
Our study showed that dynamic thiol-disulphide homoeostasis has shifted towards disulphide formation, as a result of thiol oxidation in ARC patients. The present study is the first to measure thiol-disulphide homoeostasis in ARC patients with a novel automated assay. This study supports the hypothesis that cataract is an oxidative disorder. Further studies are required in order to examine the relationship between oxidative stress and the development of cataract formation.
本研究旨在测定年龄相关性白内障（ARC）患者的血浆硫醇 - 二硫化物稳态，并将患者结果与健康受试者进行比较。还对血浆丙二醛（MDA）水平和过氧化氢酶（CAT）活性进行了研究。
该研究纳入了53例白内障患者和52名健康志愿者。使用一种新型自动化方法测定天然硫醇 - 二硫化物交换。采用Aebi所述方法测定CAT活性，使用硫代巴比妥酸法计算MDA水平。
与对照组相比，白内障患者的天然硫醇和总硫醇水平显著降低（分别为p < 0.001，p = 0.002）。白内障患者的二硫化物水平显著高于对照组（p = 0.002）。与对照组相比，白内障患者中二硫化物/天然硫醇和二硫化物/总硫醇的比率在统计学上更高（分别为p < 0.001，p < 0.001）。此外，白内障患者组的CAT活性显著低于对照组（p < 0.001），患者组的MDA水平略高但无统计学意义（p = 0.581）。
我们的研究表明，由于ARC患者中硫醇氧化，动态硫醇 - 二硫化物稳态已向二硫化物形成方向转变。本研究首次采用新型自动化检测方法测量ARC患者的硫醇 - 二硫化物稳态。本研究支持白内障是一种氧化紊乱的假说。为了研究氧化应激与白内障形成发展之间的关系，还需要进一步的研究。
