Zhang Ju-Cheng, Chen Guang-Yi, Li Xiao-Zhan, Hu Ming, Wang Bang-Yan, Ruan Bao-Hui, Zhou Hao, Zhao Li-Xing, Zhou Jun, Ding Zhong-Tao, Yang Ya-Bin
a School of Chemical Science and Technology , Yunnan University , Kunming , China.
b College of Science , Honghe University , Mengzi , China.
Nat Prod Res. 2017 Dec;31(23):2745-2752. doi: 10.1080/14786419.2017.1295235. Epub 2017 Mar 2.
A new natural mycotoxin was isolated from the fermentation broth of Trichoderma sp. Jing-8 and the structure was determined as alternariol 1'-hydroxy-9-methyl ether (1), together with twelve known compounds. The structures were elucidated on the basis of their 1D, 2D NMR spectra and mass spectrometric data. Compounds 1, 8 and 9 indicated inhibitions against germination of the seeds of cabbage with MICs < 3 μg/mL. The compound 1 showed the antibacterial activity against Bacillus subtilis and Staphylococcus aureus with MICs at 64 μg/mL. Compound 1 and 3 showed significant DPPH radical-scavenging activities with IC at 12 μg/mL, respectively. The OH at C-1' in compound 1 decreased the cytotoxicity of these mycotoxins. A primary structure-activity relationship about the alternariol derivatives was discussed. Compounds 2-7 and 8 were the first time to be isolated from the Trichoderma.
从木霉菌株Jing-8的发酵液中分离出一种新的天然霉菌毒素，其结构被确定为链格孢酚1'-羟基-9-甲醚（1），同时还分离出了12种已知化合物。通过一维、二维核磁共振光谱和质谱数据对其结构进行了阐明。化合物1、8和9对白菜种子萌发有抑制作用，最低抑菌浓度（MIC）<3μg/mL。化合物1对枯草芽孢杆菌和金黄色葡萄球菌有抗菌活性，MIC为64μg/mL。化合物1和3分别在12μg/mL时表现出显著的1,1-二苯基-2-三硝基苯肼（DPPH）自由基清除活性。化合物1中C-1'位的羟基降低了这些霉菌毒素的细胞毒性。讨论了链格孢酚衍生物的初步构效关系。化合物2-7和8首次从木霉菌中分离得到。
