Mollicellins O⁻R，从内生真菌 sp. Eef-10 中分离得到的四个新的表没食子儿茶素没食子酸酯

Mollicellins O⁻R, Four New Depsidones Isolated from the Endophytic Fungus sp. Eef-10.

机构信息

Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China.

Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou 510642, China.

出版信息

Molecules. 2018 Dec 5;23(12):3218. doi: 10.3390/molecules23123218.

DOI:10.3390/molecules23123218

PMID:30563178

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6321418/

Abstract

Four new depsidones, mollicellins O⁻R (compounds ⁻), along with three known compounds ⁻, were isolated from cultures of the fungus sp. Eef-10, an endophyte isolated from . The structures of the new compounds were elucidated by analysis of the 1D and 2D NMR and HR-ESI-MS spectra. The known compounds were identified by comparison of their spectral data with published values. Compounds ⁻ were evaluated for antibacterial activities against (sensitive and resistant strains), , , , , , and cytotoxic activities against two human cancer cell lines (HepG2 and Hela). Mollicellin H () displayed best antibacterial activity, with IC values of 5.14 µg/mL against ATCC29213 and 6.21 µg/mL against N50, MRSA, respectively. Mollicellin O () and mollicellin I () also exhibited antibacterial activities against ATCC29213 and N50. Mollicellin G () was active against both two human cancer cell lines, with IC values of 19.64 and 13.97 µg/mL while compounds and only showed cytotoxic activity against one cell line. In addition, mollicellin O () showed antioxidant activity based on DPPH radical scavenging, with an IC value of 71.92 µg/mL.

摘要

从内生真菌 sp. Eef-10 的培养物中分离得到了 4 种新的去甲二萜化合物 mollicellins O⁻R（化合物 ⁻），以及 3 种已知化合物 ⁻。通过分析 1D 和 2D NMR 和 HR-ESI-MS 光谱确定了新化合物的结构。通过比较光谱数据与已发表的值鉴定了已知化合物。对化合物 ⁻进行了抗（敏感和耐药株）、、、、、和两种人癌细胞系（HepG2 和 Hela）的细胞毒性活性的抗菌活性评估。Mollicellin H () 显示出最佳的抗菌活性，对 ATCC29213 的 IC 值为 5.14 µg/mL，对 N50、MRSA 的 IC 值分别为 6.21 µg/mL。Mollicellin O () 和 mollicellin I () 也对 ATCC29213 和 N50 表现出抗菌活性。Mollicellin G () 对两种人癌细胞系均具有活性，IC 值分别为 19.64 和 13.97 µg/mL，而化合物和仅对一种细胞系表现出细胞毒性活性。此外，mollicellin O () 基于 DPPH 自由基清除显示出抗氧化活性，IC 值为 71.92 µg/mL。