Ghanizadeh Tabriz Atabak, Mills Christopher G, Mullins John J, Davies Jamie A, Shu Wenmiao
School of Engineering and Physical Sciences, Heriot-Watt University , Edinburgh , UK.
Centre for Integrative Physiology, University of Edinburgh, Edinburgh, UK; Centre for Cardiovascular Science, University of Edinburgh, Edinburgh, UK.
Front Bioeng Biotechnol. 2017 Feb 24;5:13. doi: 10.3389/fbioe.2017.00013. eCollection 2017.
Development of a simple, straightforward 3D fabrication method to culture cells in 3D, without relying on any complex fabrication methods, remains a challenge. In this paper, we describe a new technique that allows fabrication of scalable 3D cell-laden hydrogel structures easily, without complex machinery: the technique can be done using only apparatus already available in a typical cell biology laboratory. The fabrication method involves micro dip-coating of cell-laden hydrogels covering the surface of a metal bar, into the cross-linking reagents calcium chloride or barium chloride to form hollow tubular structures. This method can be used to form single layers with thickness ranging from 126 to 220 µm or multilayered tubular structures. This fabrication method uses alginate hydrogel as the primary biomaterial and a secondary biomaterial can be added depending on the desired application. We demonstrate the feasibility of this method, with survival rate over 75% immediately after fabrication and normal responsiveness of cells within these tubular structures using mouse dermal embryonic fibroblast cells and human embryonic kidney 293 cells containing a tetracycline-responsive, red fluorescent protein (tHEK cells).
开发一种简单、直接的三维制造方法来在三维空间中培养细胞，而不依赖于任何复杂的制造方法，仍然是一项挑战。在本文中，我们描述了一种新技术，该技术能够轻松制造可扩展的载有细胞的三维水凝胶结构，无需复杂的机器设备：该技术仅使用典型细胞生物学实验室中已有的仪器即可完成。制造方法包括将覆盖在金属棒表面的载有细胞的水凝胶进行微浸涂，浸入交联试剂氯化钙或氯化钡中以形成中空管状结构。此方法可用于形成厚度范围为126至220微米的单层或多层管状结构。这种制造方法以藻酸盐水凝胶作为主要生物材料，可根据所需应用添加第二种生物材料。我们使用小鼠真皮胚胎成纤维细胞和含有四环素响应性红色荧光蛋白的人胚胎肾293细胞（tHEK细胞），证明了该方法的可行性，制造后立即存活率超过75%，且这些管状结构内的细胞具有正常反应性。
