Ihsan Muhammad Z, Ahmad Samina J N, Shah Zahid Hussain, Rehman Hafiz M, Aslam Zubair, Ahuja Ishita, Bones Atle M, Ahmad Jam N
Cholistan Institute of Desert Studies, The Islamia University Bahawalpur Bahawalpur, Pakistan.
Plant Stress Physiology and Molecular Biology Lab, Department of Botany, University of Agriculture FaisalabadFaisalabad, Pakistan; Integrated Genomics Cellular Developmental and Biotechnology Lab, Department of Entomology, University of Agriculture FaisalabadFaisalabad, Pakistan.
Front Plant Sci. 2017 Feb 27;8:233. doi: 10.3389/fpls.2017.00233. eCollection 2017.
The cell wall (CW) as a first line of defense against biotic and abiotic stresses is of primary importance in plant biology. The proteins associated with cell walls play a significant role in determining a plant's sustainability to adverse environmental conditions. In this work, the genes encoding cell wall proteins (CWPs) in Arabidopsis were identified and functionally classified using geneMANIA and GENEVESTIGATOR with published microarrays data. This yielded 1605 genes, out of which 58 genes encoded proline-rich proteins (PRPs) and glycine-rich proteins (GRPs). Here, we have focused on the cellular compartmentalization, biological processes, and molecular functioning of proline-rich CWPs along with their expression at different plant developmental stages. The mined genes were categorized into five classes on the basis of the type of PRPs encoded in the cell wall of . We review the domain structure and function of each class of protein, many with respect to the developmental stages of the plant. We have then used networks, hierarchical clustering and correlations to analyze co-expression, co-localization, genetic, and physical interactions and shared protein domains of these PRPs. This has given us further insight into these functionally important CWPs and identified a number of potentially new cell-wall related proteins in .
细胞壁（CW）作为抵御生物和非生物胁迫的第一道防线，在植物生物学中至关重要。与细胞壁相关的蛋白质在决定植物对不利环境条件的耐受性方面发挥着重要作用。在这项工作中，利用geneMANIA和GENEVESTIGATOR以及已发表的微阵列数据，对拟南芥中编码细胞壁蛋白（CWP）的基因进行了鉴定和功能分类。这产生了1605个基因，其中58个基因编码富含脯氨酸的蛋白质（PRP）和富含甘氨酸的蛋白质（GRP）。在这里，我们重点研究了富含脯氨酸的细胞壁蛋白的细胞区室化、生物学过程和分子功能，以及它们在植物不同发育阶段的表达。根据拟南芥细胞壁中编码的PRP类型，将挖掘出的基因分为五类。我们回顾了每类蛋白质的结构域结构和功能，其中许多与植物的发育阶段有关。然后，我们利用网络、层次聚类和相关性分析这些PRP的共表达、共定位、遗传和物理相互作用以及共享的蛋白质结构域。这使我们对这些功能重要的细胞壁蛋白有了进一步的了解，并在拟南芥中鉴定出了一些潜在的新的细胞壁相关蛋白。
