van Wijnen A J, Wright K L, Massung R F, Gerretsen M, Stein J L, Stein G S
Department of Cell Biology, University of Massachussetts Medical Center, Worcester 01655.
Nucleic Acids Res. 1988 Jan 25;16(2):571-92. doi: 10.1093/nar/16.2.571.
The 5' region of a cell cycle regulated human H1 histone gene appears to contain at least six promoter DNA elements that are shared with some, but not all human core (H2A, H2B, H3 and H4) histone genes. We show that two of these elements represent separate binding sites for two distinct, partially purified factors. The first promoter domain contains A/T rich repeats and is involved in the binding of HiNF-A, a nuclear factor previously found to bind to A/T rich direct repeats in the promoters of human H4 and H3 histone genes. The second domain, containing the general promoter element 5' dACCAAT, acts as a binding site for a two component mosaic factor we have designated HiNF-B. These data suggest that coordinate transcriptional regulation of human H1 and core histone genes may involve two classes of trans-acting factors: those specific for histone gene promoters and those that act on a broad spectrum of human gene promoters.
一个细胞周期调控的人类H1组蛋白基因的5'区域似乎包含至少六个启动子DNA元件，这些元件与一些但并非所有人类核心（H2A、H2B、H3和H4）组蛋白基因共有。我们表明，其中两个元件代表两个不同的、部分纯化因子的单独结合位点。第一个启动子结构域含有富含A/T的重复序列，参与HiNF-A的结合，HiNF-A是一种先前发现可与人H4和H3组蛋白基因启动子中的富含A/T的直接重复序列结合的核因子。第二个结构域包含通用启动子元件5'dACCAAT，作为我们命名为HiNF-B的双组分镶嵌因子的结合位点。这些数据表明，人类H1和核心组蛋白基因的协同转录调控可能涉及两类反式作用因子：组蛋白基因启动子特异性的因子和作用于广泛人类基因启动子的因子。
