Mirghaed Ali Taheri, Ghelichpour Melika, Hoseini Seyyed Morteza, Amini Kourosh
Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Inland Waters Aquatic Stocks Research Center, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Gorgan, Iran.
Fish Physiol Biochem. 2017 Aug;43(4):1143-1151. doi: 10.1007/s10695-017-0359-y. Epub 2017 Mar 14.
The present study aimed to determine hemolysis interference in measurement of plasma biochemical parameters in fish specimens. For this purpose, blood samples were harvested from 24 Huso huso juveniles. After centrifugation, each plasma sample was divided into seven portions to make seven levels of hemolysis. Hemolysis was induced by addition of different percentages of the corresponding whole blood [0 (non-hemolyzed control group), 1, 3, 5, 10, 15, and 20% of whole blood]. Albumin, total protein, calcium, phosphorus, glucose, sodium, potassium, chloride, alkaline phosphatase (ALP), aspartate transaminase (AST), and alanine transaminase (ALT) were measured in different samples. Results showed that plasma albumin, ALP, potassium, and AST significantly increased (more than 200% at the highest hemolysis level) in the hemolyzed samples. Also, plasma total protein and phosphorus showed significant elevation in the hemolyzed samples (more than 70% at the highest hemolysis level). Plasma glucose, calcium, chloride, and ALT showed narrow but significant increase in hemolyzed samples (11.8-35.2% at the highest hemolysis level). Plasma sodium showed no significant changes in the hemolyzed samples. In conclusion, the present results show that hemolysis markedly affects plasma parameters levels, which interferes with plasma results interpretation. Therefore, analysis of hemolyzed samples should be avoided or the results should be interpreted with caution. This study encourages further investigations to develop methods for omitting the effects of hemolysis by sample blanking and/or presenting correction coefficients for measurement of plasma parameters in samples with different levels of hemolysis.
本研究旨在确定溶血对鱼类样本血浆生化参数测量的干扰。为此，从24条小胡瓜鱼中采集血液样本。离心后，将每个血浆样本分成七份，制成七个溶血水平。通过添加不同百分比的相应全血（0（非溶血对照组）、全血的1%、3%、5%、10%、15%和20%）诱导溶血。对不同样本测量白蛋白、总蛋白、钙、磷、葡萄糖、钠、钾、氯、碱性磷酸酶（ALP）、天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）。结果表明，溶血样本中的血浆白蛋白、ALP、钾和AST显著增加（在最高溶血水平时超过200%）。此外，溶血样本中的血浆总蛋白和磷也显著升高（在最高溶血水平时超过70%）。血浆葡萄糖、钙、氯和ALT在溶血样本中虽有小幅但显著的增加（在最高溶血水平时为11.8 - 35.2%）。血浆钠在溶血样本中无显著变化。总之，目前的结果表明，溶血显著影响血浆参数水平，这会干扰血浆结果的解读。因此，应避免分析溶血样本，或者应谨慎解读结果。本研究鼓励进一步开展调查，以开发通过样本空白化和/或给出不同溶血水平样本血浆参数测量校正系数来消除溶血影响的方法。
