Department of Conservative Dentistry and Endodontics, Bharath Institute of Higher Training and Research, Tamilnadu, India.
Department of Conservative Dentistry and Endodontics, Sree Balaji Dental College and Hospital, Tamilnadu, India.
Eur Endod J. 2022 Mar;7(1):58-66. doi: 10.14744/eej.2021.70783.
The purpose of this in vitro study was to compare the antimicrobial effectiveness of 2% chlorhexidine gel, 0.5% chitosan, ethanol extract of mangosteen pericarp, and Triphala used as intracanal medicaments against Enterococcus faecalis and Candida albicans in mono- and dual-species biofilms.
Bioactive components in the ethanol extract of mangosteen pericarp and Triphala were evaluated by gas chromatography-mass spectrometry (GCMS). Quantitative assessment of the biofilm formations of E. faecalis and C. albicans and as a dual-species in the presence of test medicaments was carried out using a crystal violet (CV) assay in a microtiter plate. Following this, 246 single-rooted premolar teeth were collected, and root specimens were prepared. C. albicans and E. faecalis mono- and dual-species biofilms were grown in the root specimens. At the end of 21 days, the samples were divided into five groups and subjected to different types of medicaments: Control group- distilled water; Chlorhexidine group- 2% chlorhexidine gel; Chitosan group- 0.5% chitosan; Mangosteen group- ethanol extract of mangosteen pericarp; and Triphala group- ethanol extract of Triphala. Colony-forming units (CFUs) were assessed on the first and fifth day after medicament placement.
Microbial population reduction was measured by one-way analysis of variance, followed by post-hoc Tukey's multiple comparison test (P<0.05). Chlorhexidine showed maximum log reduction in CFUs of microorganisms, followed by chitosan, which showed a similar log reduction (P>0.05) for both mono- and dual-species biofilms. However, in the mangosteen and Triphala extract groups, the CFU/mL for dual-species on both days did not have a significant reduction in count (P<0.05) when compared to chlorhexidine and chitosan.
Chlorhexidine showed maximum antimicrobial activity, followed by chitosan, on both mono- and dual-species biofilms. Mangosteen and Triphala had good antimicrobial action on the mono-species biofilm.
本体外研究旨在比较 2%洗必泰凝胶、0.5%壳聚糖、番石榴果皮乙醇提取物和 Triphala 作为根管内药物对单种和混合生物膜中粪肠球菌和白色念珠菌的抗菌效果。
采用气相色谱-质谱联用（GCMS）法评价番石榴果皮乙醇提取物和 Triphala 的生物活性成分。采用结晶紫（CV）微量板法对粪肠球菌和白色念珠菌的生物膜形成进行定量评估，并在存在测试药物的情况下进行混合生物膜的形成。在此之后，收集了 246 颗单根前磨牙，并制备了根标本。在根标本中培养白色念珠菌和粪肠球菌单种和混合生物膜。21 天后，将样本分为五组，分别用不同类型的药物处理：对照组-蒸馏水；洗必泰组-2%洗必泰凝胶；壳聚糖组-0.5%壳聚糖；番石榴组-番石榴果皮乙醇提取物；Triphala 组- Triphala 乙醇提取物。在放置药物后的第 1 天和第 5 天评估菌落形成单位（CFU）。
采用单因素方差分析测量微生物种群减少，然后进行事后 Tukey 多重比较检验（P<0.05）。洗必泰对微生物 CFU 的对数减少最大，其次是壳聚糖，对单种和混合生物膜的对数减少相似（P>0.05）。然而，在番石榴和 Triphala 提取物组中，与洗必泰和壳聚糖相比，两种生物膜在第 2 天的混合生物膜 CFU/mL 计数没有显著减少（P<0.05）。
洗必泰对单种和混合生物膜显示出最大的抗菌活性，其次是壳聚糖。番石榴和 Triphala 对单种生物膜具有良好的抗菌作用。
