Kuznetsova D, Ageykin A, Koroleva A, Deiwick A, Shpichka A, Solovieva A, Kostjuk S, Meleshina A, Rodimova S, Akovanceva A, Butnaru D, Frolova A, Zagaynova E, Chichkov B, Bagratashvili V, Timashev P
Institute of Biomedical Technologies, Nizhny Novgorod State Medical Academy, Nizhny Novgorod, 603005, Russia. Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, 603950, Russia.
Biofabrication. 2017 Apr 28;9(2):025009. doi: 10.1088/1758-5090/aa6725.
In the presented study, we have developed a synthetic strategy allowing a gradual variation of a polylactide arms' length, which later influences the micromorphology of the scaffold surface, formed by a two-photon polymerization technique. It has been demonstrated that the highest number of cells is present on the scaffolds with the roughest surface made of the polylactide with longer arms (PLA760), and osteogenic differentiation of mesenchymal stem cells is most pronounced on such scaffolds. According to the results of biological testing, the PLA760 scaffolds were implanted into a created cranial defect in a mouse for an in vivo assessment of the bone tissue formation. The in vivo experiments have shown that, by week 10, deposition of calcium phosphate particles occurs in the scaffold at the defect site, as well as, the formation of a new bone and ingrowth of blood vessels from the surrounding tissues. These results demonstrate that the cross-linked microstructured tetrafunctional polylactide scaffolds are promising microstructures for bone regeneration in tissue engineering.
在本研究中，我们开发了一种合成策略，可使聚丙交酯臂的长度逐渐变化，这随后会影响通过双光子聚合技术形成的支架表面的微观形态。结果表明，细胞数量最多的是由较长臂的聚丙交酯（PLA760）制成的表面最粗糙的支架，间充质干细胞在这种支架上的成骨分化最为明显。根据生物学测试结果，将PLA760支架植入小鼠颅骨缺损处，以对骨组织形成进行体内评估。体内实验表明，到第10周时，缺损部位的支架中出现磷酸钙颗粒沉积，以及新骨形成和周围组织血管长入。这些结果表明，交联的微结构四官能聚丙交酯支架是组织工程中骨再生的有前景的微结构。
