来自皱盖乌芝子实体的高氧化羊毛甾烷型三萜类化合物及其生物活性。
Highly oxygenated lanostane-type triterpenoids and their bioactivity from the fruiting body of Ganoderma gibbosum.
作者信息
Pu De-Bing, Zheng Xi, Gao Jun-Bo, Zhang Xing-Jie, Qi Yan, Li Xiao-Si, Wang Yong-Mei, Li Xiao-Nian, Li Xiao-Li, Wan Chun-Ping, Xiao Wei-Lie
机构信息
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
Central Laboratory, The No. 1 Affiliated Hospital of Yunnan University of Traditional Chinese Medicine, Kunming 650021, PR China.
出版信息
Fitoterapia. 2017 Jun;119:1-7. doi: 10.1016/j.fitote.2017.03.007. Epub 2017 Mar 11.
Eight new highly oxygenated lanostane triterpenes, gibbosic acids A-H (1-8), along with three known ones (9-11), were isolated from the fruiting body of Ganoderma gibbosum. The structures of new isolates were assigned by NMR and HRESIMS experiments. The absolute configurations of 1 were further confirmed by single crystal X-ray diffraction data and computational ECD methods. Immunoregulatory effect and anti-inflammatory activities of these compounds were screened in murine lymphocyte proliferation assay and in lipopolysaccharide (LPS)-stimulated RAW-264.7 macrophages, respectively. Compound 2 exhibited immunostimulatory effect both in lymphocyte proliferation assay without any induction and ConA-induced mitogenic activity of T-lymphocyte, and the proportion of lymphocyte proliferation at the concentration of 0.1μM are 20.01% and 21.40%, respectively.
从密纹灵芝子实体中分离得到8个新的高氧化羊毛甾烷三萜类化合物，即赤酸A-H（1-8），以及3个已知化合物（9-11）。通过核磁共振（NMR）和高分辨电喷雾电离质谱（HRESIMS）实验确定了新分离化合物的结构。通过单晶X射线衍射数据和计算电子圆二色光谱（ECD）方法进一步确定了化合物1的绝对构型。分别在小鼠淋巴细胞增殖试验和脂多糖（LPS）刺激的RAW-264.7巨噬细胞中筛选了这些化合物的免疫调节作用和抗炎活性。化合物2在无任何诱导的淋巴细胞增殖试验和刀豆蛋白A（ConA）诱导的T淋巴细胞促有丝分裂活性中均表现出免疫刺激作用，在0.1μM浓度下淋巴细胞增殖比例分别为20.01%和21.40%。
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