New lanostane-type triterpenoids with proangiogenic activity from the fruiting body of .

Two new lanostane-type triterpenoids, ganoderenicfys A () and B (), together with six related known terpenoids (-), were isolated and identified from the fruiting body of . The structures of these compounds were established on the basis of detailed interpretation of their NMR and HRESIMS data. The absolute configurations of and were determined by quantum chemical electronic circular dichroism (ECD) calculations. All of the isolated compounds were evaluated for their proangiogenic activities in a transgenic fluorescent zebrafish model. Compounds - displayed dose-dependently proangiogenic activity in a PTK787-induced vascular injury zebrafish model, while compounds , and significantly promoted the angiogenesis. This is the first report for proangiogenic activities of lanostane-type triterpenoids.