Yasugi Sadao, Mizuno Takeo
The Zoological Institute, University of Tokyo, Tokyo, Japan.
Wilhelm Roux Arch Entwickl Mech Org. 1974 Jun;174(2):107-116. doi: 10.1007/BF00573624.
Dissociation and reassociation experimentsin vitro were carried out to investigate the differentiation potency of chick allantoic endoderm under the influence of digestive tract mesenchymes. 1. The allantoic endoderm, when cultured alonein vitro, shows no differentiation whatsoever. 2. The allantoic endoderm, when cultivated combined with the mesenchyme of oesophagus, can differentiate into a stratified cuboidal epithelium, similar to that of the embryonic oesophagus. 3. Cultivation of the allantoic endoderm combined with the proventricular mesenchyme causes differentiation of cylindrical epithelium and glands, which are characteristic of the embryonic proventriculus. 4. The combination of the allantoic endoderm and the gizzard mesenchyme results in the differentiation of pseudostratified columnar epithelium, the cells of which possess glycogen granules like those in the normal embryonic gizzard. 5. If the allantoic endoderm is cultured on the mesenchyme of the small intestine, the endodermal cells are converted into simple columnar epithelial cells similar to those of normal embryonic small intestine. 6. The competence for the heterotypic development of the allantoic endoderm appears to be a function of a developmental time sequence: it is highest in the youngest (3-day) allantoic endoderm, and gradually lost in older embryos. 7. In all combinations tested, there appear goblet cells in the epithelium when the explants are cultured more than 10 days. These cells are never observed in intact oesophagus, proventriculus and gizzard, whether in normal development or in culture.
进行了体外解离和重新结合实验，以研究在消化道间充质影响下鸡尿囊内胚层的分化潜能。1. 尿囊内胚层在体外单独培养时，未显示出任何分化。2. 尿囊内胚层与食管间充质联合培养时，可分化为复层立方上皮，类似于胚胎食管的上皮。3. 尿囊内胚层与前胃间充质联合培养导致圆柱状上皮和腺体分化，这是胚胎前胃的特征。4. 尿囊内胚层与肌胃间充质结合导致假复层柱状上皮分化，其细胞具有与正常胚胎肌胃中细胞相似的糖原颗粒。5. 如果尿囊内胚层在小肠间充质上培养，内胚层细胞会转化为类似于正常胚胎小肠的单层柱状上皮细胞。6. 尿囊内胚层异型发育的能力似乎是发育时间序列的函数：在最年轻（3天）的尿囊内胚层中最高，并在较老的胚胎中逐渐丧失。7. 在所有测试的组合中，当外植体培养超过10天时，上皮中会出现杯状细胞。在正常发育或培养的完整食管、前胃和肌胃中从未观察到这些细胞。
